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首页> 外文期刊>Experimental Neurology >Exercise modulates microRNAs that affect the PTEN/mTOR pathway in rats after spinal cord injury
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Exercise modulates microRNAs that affect the PTEN/mTOR pathway in rats after spinal cord injury

机译：运动调节影响脊髓损伤后大鼠PTEN / mTOR途径的microRNA

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摘要

We investigated microRNAs (miRs) associated with PTEN/mTOR signaling after spinal cord injury (SCI) and after hind limb exercise (Ex), a therapy implicated in promoting spinal cord plasticity. After spinalization, rats received cycling Ex 5. days/week. The expression of miRs, their target genes and downstream effectors were probed in spinal cord tissue at 10 and 31. days post injury. Ex elevated expression of miR21 and decreased expression of miR 199a-3p correlating with significant change in the expression of their respective target genes: PTEN mRNA decreased and mTOR mRNA increased. Western blotting confirmed comparable changes in protein levels. An increase in phosphorylated-S6 (a downstream effector of mTOR) within intermediate grey neurons in Ex rats was blocked by Rapamycin treatment. It thus appears possible that activity-dependent plasticity in the injured spinal cord is modulated in part through miRs that regulate PTEN and mTOR signaling and may indicate an increase in the regenerative potential of neurons affected by a SCI.
机译：我们研究了与脊髓损伤（SCI）和后肢运动（Ex）后的PTEN / mTOR信号传导相关的microRNA（miRs），这是一种促进脊髓可塑性的疗法。脊柱化后，大鼠接受Ex 5.天/周的循环。在损伤后第10和31天在脊髓组织中探查miR，它们的靶基因和下游效应子的表达。 miR21的前表达升高和miR 199a-3p的降低表达与它们各自靶基因表达的显着变化相关：PTEN mRNA降低而mTOR mRNA升高。蛋白质印迹证实了蛋白质水平的可比变化。雷帕霉素治疗可抑制Ex大鼠中间灰色神经元内磷酸化S6（mTOR的下游效应子）的增加。因此，似乎有可能通过调节PTEN和mTOR信号传导的miR来部分调节受损脊髓中的活动依赖性可塑性，并可能表明受SCI影响的神经元的再生潜力增加。

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