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Introduction to Festschrift/Special Issue: Normal and abnormal neuronal oscillations in sensorimotor pathways

机译:Festschrift简介/特刊:感觉运动通路中的正常和异常神经元振荡

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Production of Z-type farnesyl diphosphate (FPP) has not been reported in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,. E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,. E-FPP synthase (Rv1086), which can produce primarily Z,. E-FPP rather than E,. E-FPP, the predominant stereoisomer found in most organisms. Z,. E-farnesol (FOH) was produced from E. coli harboring the bottom portion of the MVA pathway and the fusion FPP synthase (ILRv) at a titer of 115.6. mg/L in 2. YT medium containing 1% (v/v) glycerol as a carbon source and 5. mM mevalonate. The Z,E-FOH production was improved by 15-fold, compared with 7.7. mg/L obtained from the co-overexpression of separate IspA and Rv1086. The Z,. E-FPP was not metabolized in native metabolic pathways of E. coli. It would be of interest to produce Z,. E-FPP derived sesquiterpenes from recombinant E. coli due to no loss of Z,. E-FPP substrate in endogenous metabolism of the host strain.
机译:Z型法呢基二磷酸酯(FPP)的生产尚未在大肠杆菌中报道。在这里,我们介绍了大肠杆菌E.的融合酶(ILRv)。 E-FPP合酶(IspA)和结核分枝杆菌Z。 E-FPP合酶(Rv1086),可以主要产生Z。 E-FPP,而不是E。 E-FPP,在大多数生物中发现的主要立体异构体。 Z,从具有MVA途径底部的E. coli和融合FPP合酶(ILRv)以115.6的效价生产E-法尼醇(FOH)。在含有1%(v / v)甘油作为碳源和5. mM甲羟戊酸的2. YT培养基中,mg / L。与7.7相比,Z,E-FOH的产量提高了15倍。从单独的IspA和Rv1086的共过表达获得的mg / L。 Z, E-FPP未在大肠杆菌的天然代谢途径中代谢。产生Z,将是令人感兴趣的。由于没有丢失Z,E-FPP衍生自重组大肠杆菌的倍半萜。 E-FPP底物在宿主菌株的内源性代谢中。

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