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首页> 外文期刊>Experimental dermatology >Somatostatin regulates tight junction function and composition in human keratinocytes.
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Somatostatin regulates tight junction function and composition in human keratinocytes.

机译：生长抑素调节人角质形成细胞中的紧密连接功能和组成。

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摘要

Somatostatin (SST) is a regulatory peptide hormone that acts through five different G protein-coupled receptors (SSTR1-5). Whereas expression of all five SSTR subtypes in epidermis has been shown, the biological relevance of the SST/SSTR system in the skin is completely unknown. We show here that SST is expressed in human skin and is present in a subset of Merkel cells and dendritic cells as well as in keratinocytes. We focused further on the somatostatin receptor subtype 3 (SSTR3) and its interacting protein MUPP1, as both were found to be localized at cellular junctions in epidermal keratinocytes. MUPP1 is a component of tight junctions (TJs); these cell-cell junctions contribute to barrier function of the paracellular pathway in cultured keratinocytes. We provide evidence that SSTR3 and MUPP1 interact in primary cultured human keratinocytes at high Ca(2+) conditions. Interestingly, SST, presumably via SSTR3/MUPP1, regulates TJ permeability in cultured keratinocytes. During long-term treatment of human keratinocytes, SST also affects the expression of distinct TJ proteins such as claudin-4. Our data are the first example of a peptide hormone regulating TJ functionality and composition in human keratinocytes, suggesting that control via peptide hormones provides the possibility to regulate the TJ barrier characteristics of the skin.
机译：生长抑素（SST）是一种调节肽激素，可通过五个不同的G蛋白偶联受体（SSTR1-5）发挥作用。尽管表皮中所有五种SSTR亚型均已表达，但皮肤中SST / SSTR系统的生物学相关性却完全未知。我们在这里显示SST在人类皮肤中表达，并存在于默克尔细胞和树突状细胞以及角质形成细胞中。我们进一步关注生长抑素受体亚型3（SSTR3）及其相互作用蛋白MUPP1，因为两者均位于表皮角质形成细胞的细胞交界处。 MUPP1是紧密连接（TJ）的组件;这些细胞间连接有助于培养的角质形成细胞中旁细胞途径的屏障功能。我们提供的证据表明，SSTR3和MUPP1在高Ca（2+）条件下在原代培养的人类角质形成细胞中相互作用。有趣的是，SST可能通过SSTR3 / MUPP1来调节培养的角质形成细胞的TJ通透性。在人类角质形成细胞的长期治疗期间，SST还影响独特的TJ蛋白（例如claudin-4）的表达。我们的数据是调节人角质形成细胞中TJ功能和组成的肽激素的第一个例子，表明通过肽激素进行控制提供了调节皮肤TJ屏障特性的可能性。

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