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首页> 外文期刊>Biochemical Genetics >Sensitivity of translation initiation factor eIF1 as a molecular target of salt toxicity to sodic-alkaline stress in the halophytic grass leymus chinensis
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Sensitivity of translation initiation factor eIF1 as a molecular target of salt toxicity to sodic-alkaline stress in the halophytic grass leymus chinensis

机译:翻译起始因子eIF1作为盐生植物对盐生草羊草的碱碱性胁迫的分子靶标的敏感性

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摘要

Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed in most organisms under normal conditions, but the transcription patterns differ under sodic-saline and sodic-alkaline stresses. Sodic-saline stress induced a persistent decrease, and sodic-alkaline stress induced overexpression of LceIF1. Potassic-saline and alkaline stresses did not cause any changes in expression of eIF1. These results indicate that not only pH but also Na+ concentration affects overtranscription of LceIF1. The eIF1 transgenic lines showed relatively high eIF1 expression, resulting in potentially higher stress resistance. Combined with eIF1 transcription in transgenic lines, LceIF1 as a molecular target of salt toxicity is believed to help enhance salt tolerance.
机译:真核翻译起始因子(eIFs)已被证明在蛋白质合成起始中至关重要。在这里,我们报告了一个潜在的有趣的牧草羊草(Leymus chinensis)(Trin。)的一个新基因LceIF1的克隆和鉴定。表达结果表明,LceIF1在大多数生物体中在正常条件下表达,但其转录模式在钠盐和钠碱胁迫下有所不同。钠盐胁迫导致持续减少,而钠盐胁迫导致LceIF1的过度表达。钾盐胁迫和碱性胁迫不会引起eIF1表达的任何变化。这些结果表明,不仅pH而且Na +浓度也影响LceIF1的过度转录。 eIF1转基因品系显示相对较高的eIF1表达,导致潜在的较高抗逆性。与转基因品系中的eIF1转录结合,LceIF1作为盐毒性的分子靶标被认为有助于增强盐耐受性。

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