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首页> 外文期刊>Biochemical Genetics >Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
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Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies

机译:使用FTA卡收集的血液,分子标记物和形态鉴定涂有油漆和乳白色的鹳的杂种

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摘要

Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird's DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.
机译:在马来西亚的一家动物园中发现了彩绘鹳和乳鹳的可疑杂种。这些鸟的血液在FTA卡上采样以进行DNA指纹识别。在44种优化引物中,有6种产生了可鉴定杂种的诊断标记。标记基于简单,直接的PCR生成的多位点条带模式,该模式提供了两组遗传数据,一组用于两种鹳,另一组用于杂交。它还显示,可以从FTA卡上收集的血液中扩增出大的DNA片段(3,000 bp)。当将每只鸟的DNA指纹图谱的结果与羽毛特征进行比较时,发现这些杂种表现出一系列纯种的中间表型性状,而没有任何亲本物种的主要羽毛特征。

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