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首页> 外文期刊>Experimental and clinical endocrinology and diabetes: Official journal, German Society of Endocrinology [and] German Diabetes Association >Liver lipocalin 2 expression in severely obese women with non alcoholic fatty liver disease
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Liver lipocalin 2 expression in severely obese women with non alcoholic fatty liver disease

机译：严重肥胖非酒精性脂肪肝妇女的肝脏脂质运载蛋白2表达

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Background: Lipocalin 2 (LCN2) has been related to obesity, insulin resistance and metabolic disturbance. However, its relation with non alcoholic fatty liver disease (NAFLD) has hardly been studied. Methods: We examined LCN2 circulating levels and its protein and gene expression in liver from women with severe obesity and NAFLD. We analyzed the liver histology of 59 white severely obese women (BMI ≥40 Kg/m2): 15 subjects presented normal liver histology or non-significant liver disease (NL), 18 simple steatosis (SS) and 26 non alcoholic steatohepatitis (NASH). We determined the anthropometric and metabolic features of the women. LCN2 levels were determined by an ELISA and liver mRNA expression by real time RT-PCR. We also studied LCN2 expression in HepG2 liver cells under various inflammatory stimuli. Results: Liver LCN2 protein and gene expression were higher in NAFLD than in obese with NL. Liver LCN2 gene expression correlated with SS (r=0.351, p=0.016), and its protein expression correlated with NASH (r=0.705, p=0.003). LCN2 expression was detected in HepG2 cells after the administration of TNFα, IL6, resistin or adiponectin. LCN2 expression was induced by TNFα, IL6 and resistin. Conclusions: Liver LCN2 is related to NAFLD in severely obese women. Up-regulation of LCN2 expression is detected in HepG2 cells after exposure to TNFα, IL6 and resistin. These results suggest that LCN2 expression is induced under liver harmful conditions.
机译：背景：Lipocalin 2（LCN2）与肥胖，胰岛素抵抗和代谢紊乱有关。但是，其与非酒精性脂肪肝疾病（NAFLD）的关系尚未得到研究。方法：我们检查了重度肥胖和NAFLD妇女肝脏中LCN2循环水平及其蛋白和基因表达。我们分析了59名白人严重肥胖妇女（BMI≥40 Kg / m2）的肝组织学：15名受试者表现出正常的肝组织学或非重大肝病（NL），18名单纯脂肪变性（SS）和26名非酒精性脂肪性肝炎（NASH） 。我们确定了妇女的人体测量和代谢特征。通过ELISA确定LCN2水平，通过实时RT-PCR确定肝脏mRNA表达。我们还研究了在各种炎症刺激下HepG2肝细胞中LCN2的表达。结果：NAFLD的肝脏LCN2蛋白和基因表达高于NL肥胖者。肝LCN2基因表达与SS相关（r = 0.351，p = 0.016），其蛋白质表达与NASH相关（r = 0.705，p = 0.003）。给予TNFα，IL6，抵抗素或脂联素后，在HepG2细胞中检测到LCN2表达。 LCN2表达是由TNFα，IL6和抵抗素诱导的。结论：严重肥胖妇女肝脏LCN2与NAFLD有关。暴露于TNFα，IL6和抵抗素后，在HepG2细胞中检测到LCN2表达的上调。这些结果表明，LCN2表达在肝脏有害条件下被诱导。

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