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Cloning of the Xuhuai goat PPARγ gene and the preparation of transgenic sheep

机译：徐淮山羊PPARγ基因的克隆及转基因绵羊的制备

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This study aimed to clone the peroxisome proliferator-activated receptor γ (PPARγ) gene of the Xuhuai goat and to make transgenic sheep using intratesticular injection, so as to improve the meat quality and flavor by increasing the intramuscular fat content. The coding sequence of the goat PPARγ gene was 1,428 bp, encoding 475 amino acids. Its similarity with other species was 81 (chicken), 89 (mouse), 92 (pig), 98 (cow), and 99% (sheep). The similarity of the corresponding amino acid sequences was 92.9, 97.3, 98.3, 99.6, and 99.8%, respectively. The signal peptide region of the PPARγ protein was not found in this study, demonstrating that the protein is not secreted. RT-PCR and western blot revealed that PPARγ was expressed in vitro, and the protein was localized in the cytoplasm. The PPARγ gene was expressed in F1 transgenic sheep at both the mRNA and the protein levels; the positive ratio was 13.7%.
机译：本研究旨在克隆徐淮山羊过氧化物酶体增殖物激活受体γ（PPARγ）基因，并通过睾丸内注射法制备转基因绵羊，从而通过增加肌内脂肪含量来改善肉质和风味。山羊PPARγ基因的编码序列为1,428 bp，编码475个氨基酸。它与其他物种的相似性为81（鸡），89（小鼠），92（猪），98（牛）和99％（绵羊）。相应氨基酸序列的相似性分别为92.9、97.3、98.3、99.6和99.8％。在这项研究中未发现PPARγ蛋白的信号肽区域，表明该蛋白未被分泌。 RT-PCR和Western blot显示PPARγ在体外表达，且该蛋白定位于细胞质中。 PPARγ基因在F1转基因绵羊中的mRNA和蛋白水平均表达。阳性率为13.7％。

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