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首页> 外文期刊>Experimental Eye Research >Imaging the aqueous humor outflow pathway in human eyes by three-dimensional micro-computed tomography (3D micro-CT).
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Imaging the aqueous humor outflow pathway in human eyes by three-dimensional micro-computed tomography (3D micro-CT).

机译：通过三维微计算机断层扫描（3D micro-CT）对人眼中房水流出路径进行成像。

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The site of outflow resistance leading to elevated intraocular pressure in primary open-angle glaucoma is believed to be located in the region of Schlemm's canal inner wall endothelium, its basement membrane and the adjacent juxtacanalicular tissue. Evidence also suggests collector channels and intrascleral vessels may have a role in intraocular pressure in both normal and glaucoma eyes. Traditional imaging modalities limit the ability to view both proximal and distal portions of the trabecular outflow pathway as a single unit. In this study, we examined the effectiveness of three-dimensional micro-computed tomography (3D micro-CT) as a potential method to view the trabecular outflow pathway. Two normal human eyes were used: one immersion fixed in 4% paraformaldehyde and one with anterior chamber perfusion at 10 mmHg followed by perfusion fixation in 4% paraformaldehyde/2% glutaraldehyde. Both eyes were postfixed in 1% osmium tetroxide and scanned with 3D micro-CT at 2 mum or 5 mum voxel resolution. In the immersion fixed eye, 24 collector channels were identified with an average orifice size of 27.5 +/- 5 mum. In comparison, the perfusion fixed eye had 29 collector channels with a mean orifice size of 40.5 +/- 13 mum. Collector channels were not evenly dispersed around the circumference of the eye. There was no significant difference in the length of Schlemm's canal in the immersed versus the perfused eye (33.2 versus 35.1 mm). Structures, locations and size measurements identified by 3D micro-CT were confirmed by correlative light microscopy. These findings confirm 3D micro-CT can be used effectively for the non-invasive examination of the trabecular meshwork, Schlemm's canal, collector channels and intrascleral vasculature that comprise the distal outflow pathway. This imaging modality will be useful for non-invasive study of the role of the trabecular outflow pathway as a whole unit.
机译：据信导致原发性开角型青光眼的眼内压升高的流出阻力部位位于施累姆氏管内壁内皮，其基底膜和邻近的颈小管区域。证据还表明，在正常和青光眼中，收集器通道和巩膜内血管可能在眼压中起作用。传统的成像方式限制了将小梁流出途径的近端和远端部分视为一个单元的能力。在这项研究中，我们检查了三维微计算机断层扫描（3D micro-CT）作为查看小梁流出途径的潜在方法的有效性。使用了两只正常的人眼：一只浸没在4％多聚甲醛中，另一只浸有10 mmHg的前房灌注，然后灌注固定在4％多聚甲醛/ 2％戊二醛中。将两只眼睛后固定在1％四氧化中，并用3D微型CT在2微米或5微米体素分辨率下进行扫描。在浸入式固定眼中，确定了24个收集器通道，平均孔口尺寸为27.5 +/- 5微米。相比之下，灌注固定眼具有29个收集器通道，平均孔口大小为40.5 +/- 13微米。收集器通道未均匀分布在眼周周围。浸入式眼与灌注式眼的施莱姆管长度没有显着差异（33.2对35.1 mm）。通过相关的光学显微镜确认了通过3D micro-CT识别的结构，位置和尺寸测量结果。这些发现证实了3D micro-CT可有效地用于构成远端流出通路的小梁网，Schlemm管，收集器通道和巩膜内脉管系统的无创检查。这种成像方式对于小梁流出途径作为一个整体的作用的非侵入性研究将是有用的。

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