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首页> 外文期刊>Experimental Eye Research >A comparative analysis of C57BL/6J and 6N substrains; chemokine/cytokine expression and susceptibility to laser-induced choroidal neovascularization
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A comparative analysis of C57BL/6J and 6N substrains; chemokine/cytokine expression and susceptibility to laser-induced choroidal neovascularization

机译：C57BL / 6J和6N亚菌株的比较分析;趋化因子/细胞因子的表达及其对激光诱导脉络膜新生血管形成的敏感性

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Age-related macular degeneration (AMD) is the most prevalent cause of blindness in the elderly. To study potential underlying mechanisms of AMD, animal models are utilized, focusing mostly on mice. Recently, genomic and phenotypic differences between the so-called control substrains, C57BL/6J and C57BL/6N, have been described in models of ocular and non-ocular diseases. In particular, the rd8 mutation of the Crb 1 gene present in the C57BL/6N has been shown to impact certain ocular phenotypes and appears to augment phenotypes generally associated with inflammation. Here, we investigated angiogenic factor and cytokine expression using pathway arrays as well as the susceptibility to laser-induced choroidal neovascularization (CNV), a model of wet AMD, in the two substrains. Age-matched 3-month-old C57BL/ 6J and C57BL/6N animals differed in gene expression levels for angiogenic factors and cytokines, with 6N animals expressing higher levels of inflammatory markers than 6Js. Yet laser-induced CNV was comparable in size between the two substrains. This lack of difference in CNV size was correlated with a gene expression profile that was comparable between the two substrains, due to the fact that the degree of change in gene expression of inflammatory markers after CNV was blunted in 6N mice. In summary, significant gene expression differences exist between C57BL/6J and C57BL/6N animals, reinforcing the notion that appropriate litter-mate controls or genetic background controls need to be used. Contrary to our expectation, CNV was not augmented in 6N animals, suggesting that low chronic inflammation in the RPE might provide a level of pre-conditioning and protection against stress. Published by Elsevier Ltd.
机译：与年龄有关的黄斑变性（AMD）是老年人失明的最普遍原因。为了研究AMD的潜在潜在机制，使用了动物模型，主要关注小鼠。最近，在眼和非眼疾病模型中已经描述了所谓的对照亚菌株C57BL / 6J和C57BL / 6N之间的基因组和表型差异。特别是，已显示出C57BL / 6N中存在的Crb 1基因的rd8突变会影响某些眼表型，并似乎会增强通常与炎症相关的表型。在这里，我们研究了使用通路阵列的血管生成因子和细胞因子表达，以及在两个亚菌株中对湿性AMD模型激光诱导的脉络膜新血管形成（CNV）的敏感性。与年龄匹配的3个月大的C57BL / 6J和C57BL / 6N动物的血管生成因子和细胞因子的基因表达水平有所不同，其中6N动物的炎症标志物水平高于6Js。然而，激光诱导的CNV在两个亚菌株之间的大小可比。 CNV大小差异的这种缺乏与两个亚菌株之间可比较的基因表达谱相关，这是由于在6N小鼠中CNV变钝后炎症标志物基因表达的变化程度。总而言之，C57BL / 6J和C57BL / 6N动物之间存在明显的基因表达差异，强化了这样的观念，即需要使用适当的同窝伴侣对照或遗传背景对照。与我们的预期相反，在6N动物中CNV并未增加，这表明RPE中的低度慢性炎症可能会提供一定水平的预处理并提供抗压力保护。由Elsevier Ltd.发布

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