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首页> 外文期刊>Experimental Gerontology >Delayed neurodegeneration and early astrogliosis after excitotoxicity to the aged brain.
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Delayed neurodegeneration and early astrogliosis after excitotoxicity to the aged brain.

机译：对老年大脑的兴奋性中毒后延迟神经变性和早期星形胶质变。

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Excitotoxicity is well recognised as a mechanism underlying neuronal cell death in several brain injuries. To investigate age-dependent differences in neurodegeneration, edema formation and astrogliosis, intrastriatal N-methyl-d-aspartate injections were performed in young (3 months) and aged (22-24 months) male Wistar rats. Animals were sacrificed at different times between 12h and 14 days post-lesion (DPL) and cryostat sections were processed for Toluidine blue, Fluoro-Jade B staining, NeuN and GFAP immunohistochemistry. Our results show that both size of tissue injury and edema were reduced in the old subjects only up to 1DPL, correlating with a slower progression of neurodegeneration with peak numbers of degenerating neurons at 3DPL in the aged, contrasting with maximum neurodegeneration at 1DPL in the young. However, old animals showed an earlier onset of astroglial response, seen at 1DPL, and a larger area of astrogliosis at all time-points studied, including a greater glial scar. In conclusion, after excitotoxic striatal damage, progression of neurodegeneration is delayed in the aged but the astroglial response is earlier and exacerbated. Our results emphasize the importance of using aged animals and several survival times for the study of acute age-related brain insults.
机译：兴奋性毒性是几种脑损伤中神经元细胞死亡的潜在机制。为了研究神经变性，水肿形成和星形胶质细胞变性的年龄依赖性差异，在幼年（3个月）和年老（22-24个月）雄性Wistar大鼠中进行了纹状体内N-甲基-d-天冬氨酸注射。在病变（DPL）后12h和14天之间的不同时间处死动物，并对低温恒温器切片进行甲苯胺蓝，Fluoro-Jade B染色，NeuN和GFAP免疫组织化学分析。我们的研究结果表明，老年受试者的组织损伤和水肿均得到降低，仅达到1DPL，这与老年人3DPL处神经退行性变的高峰和3DPL变性神经元的峰值有关，而年轻人则与1DPL时最大的神经退行性变有关。 。但是，老动物在1DPL时表现出较早的星形胶质反应发生，并且在所有研究的时间点上都有较大的星形胶质化区域，包括较大的神经胶质瘢痕。总之，兴奋性纹状体损伤后，老年人神经变性的进展被延迟，但星形胶质细胞反应较早并加剧。我们的结果强调了使用老年动物和几个存活时间来研究与年龄相关的急性脑损伤的重要性。

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