...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Burns: Including Thermal Injury >Effects of Panax notoginseng saponins on myocardial Gsalpha mRNA expression and ATPase activity after severe scald in rats.
【24h】

Effects of Panax notoginseng saponins on myocardial Gsalpha mRNA expression and ATPase activity after severe scald in rats.

机译：三七总皂甙对严重烫伤后大鼠心肌Gsalpha mRNA表达和ATPase活性的影响。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to investigate the changes of myocardial Gsalpha mRNA expression and ATPase and the effects of Panax notoginseng saponins (PNS) on that after burns in rats. Wistar rats were exposed to a 95 degrees C water bath for 10s to produce 30% TBSA skin-full-thickness scalds. Myocardial Gsalpha mRNA level, cAMP content and adenyl cyclase (AC) activities were determined with dot blotting hybridization, radioimmunoassay and indirect method, respectively. The ATPase activities in plasma membrane of cardiomyocytes and erythrocytes were measured colorimetrically. At 3, 6, 9 hours after scalds, the myocardial Gsalpha mRNA expression decreased significantly (P<0.01). PNS (100, 200mgkg(-1), i.p.) markedly increased these levels (P<0.01). The elevation was correlated significantly with PNS dose (r=0.95, P<0.05). At 3, 6, 9, 12, 24, 48 hour after the scalds, the myocardial cAMP content and AC basal activity was reduced significantly. PNS (100, 200mgkg(-1), i.p.) increased cAMP content and enhanced AC activity markedly in comparison with the 3rd hour postburn group. The activities of (Ca(2+)-Mg(2+))-ATPase and (Na(+)-K(+))-ATPase in plasma membrane of myocardial cells and red blood cells in scald group were significantly lower than those in the normal control group (P<0.01). PNS (100mgkg(-1), i.p.) improved these indexes significantly after scalds (P<0.01 or 0.05). These data suggested that the effects of PNS on myocardium in burned rats involved its action to increase myocardial Gsalpha mRNA expression and AC activity, cAMP content as well as ATPase activities.
机译：这项研究的目的是调查大鼠烧伤后心肌Gsalpha mRNA表达和ATPase的变化以及三七总皂甙（PNS）对心肌的影响。将Wistar大鼠置于95摄氏度的水浴中10秒钟，以产生30％TBSA皮肤全层烫伤。斑点印迹杂交，放射免疫法和间接法分别测定了心肌的Gsalpha mRNA水平，cAMP含量和腺苷酸环化酶（AC）活性。比色法检测心肌细胞和红细胞质膜中的ATPase活性。烫伤后3、6、9小时，心肌Gsalpha mRNA表达显着下降（P <0.01）。 PNS（100，200mgkg（-1），i.p.）明显增加这些水平（P <0.01）。升高与PNS剂量显着相关（r = 0.95，P <0.05）。烫伤后3、6、9、12、24、48小时，心肌cAMP含量和AC基础活性显着降低。与烧伤后第3小时相比，PNS（100，200mgkg（-1），i.p.）可明显增加cAMP含量并增强AC活性。烫伤组心肌细胞和红细胞的膜Ca（2 +）-Mg（2 +）-ATPase和（Na（+）-K（+））-ATPase活性明显低于正常对照组（P <0.01）。烫伤后PNS（100mgkg（-1），i.p.）显着改善了这些指标（P <0.01或0.05）。这些数据表明，PNS对烧伤大鼠心肌的作用涉及其增加心肌Gsalpha mRNA表达和AC活性，cAMP含量以及ATPase活性的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号