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首页> 外文期刊>Experimental Gerontology >Effect of melatonin on vascular responses in aortic rings of aging rats.
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Effect of melatonin on vascular responses in aortic rings of aging rats.

机译：褪黑素对衰老大鼠主动脉环血管反应的影响。

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In old animals a marked reduction in endothelium-dependent relaxation occurs. Since there is evidence that the endothelial dysfunction associated with aging may be partly related to the local formation of reactive oxygen species, the purpose of this study was to examine the effect of the natural antioxidant melatonin (10(-5)mol/l) on in vitro contractility of aged aortic rings under conditions of increased oxidative stress (40 m mol/l glucose concentration in medium). Experiments were carried out in 18-20 months old, Wistar male rats, using adult (6-7 months old) animals as controls. A higher plasma lipid peroxidation was found in aged rats as compared to the younger ones. In a first experiment, dose-response curves for acetylcholine-induced relaxation of aortic rings were conducted. Analyzed as a main factor in a factorial ANOVA, age decreased and melatonin augmented the relaxing response to acetylcholine. melatonin's restoring effect on aortic ring relaxation was found in aged aortic rings only and was more pronounced in the presence of a high glucose medium. In a second experiment, the effect of melatonin on the contractility response to phenylephrine of intact or endothelium-denuded aortic rings obtained from aged or control rats was examined in normal or high glucose medium. A main factor analysis in the factorial ANOVA indicated that age and operation augmented, and melatonin decreased, aortic ring contractility response to phenylephrine. Melatonin's restoring effect on aortic contractility was seen in aged aortic rings. The effect of age or a high glucose medium on phenylephrine-induced contractility was more pronounced in the absence of an intact endothelium. Aging did not affect the relaxant response of intact or endothelium-denuded rings to sodium nitroprusside. The results support the improvement by melatonin of vascular response in aging rats, presumably via its antioxidant activity.
机译：在老年动物中，内皮依赖性舒张发生明显降低。由于有证据表明与衰老相关的内皮功能障碍可能部分与活性氧的局部形成有关，因此本研究的目的是研究天然抗氧化剂褪黑激素（10（-5）mol / l）对氧化应激增加（培养基中葡萄糖浓度为40 m mol / l）的条件下，老年主动脉环的体外收缩性。实验是在18-20个月大的Wistar雄性大鼠中进行的，使用成年（6-7个月大）的动物作为对照。与年幼的大鼠相比，老年大鼠的血浆脂质过氧化更高。在第一个实验中，绘制了乙酰胆碱引起的主动脉环松弛的剂量反应曲线。分析为阶乘方差分析的主要因素，年龄下降，褪黑激素增强了对乙酰胆碱的松弛反应。褪黑素对主动脉环松弛的恢复作用仅在衰老的主动脉环中发现，并且在高葡萄糖培养基存在下更为明显。在第二个实验中，在正常或高葡萄糖培养基中检查了褪黑激素对从衰老或对照大鼠获得的完整或内皮剥除的主动脉环对去氧肾上腺素的收缩反应的影响。阶乘方差分析中的一项主要因素分析表明，年龄和手术时间增加，褪黑激素下降，主动脉环对去氧肾上腺素的反应。在衰老的主动脉环中可见褪黑激素对主动脉收缩力的恢复作用。在没有完整内皮的情况下，年龄或高葡萄糖培养基对苯肾上腺素诱导的收缩力的影响更为明显。衰老不影响完整的或内皮剥脱的环对硝普钠的松弛反应。这些结果支持褪黑素改善衰老大鼠血管反应的作用，大概是通过其抗氧化活性。

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