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首页> 外文期刊>Experimental Gerontology >Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism.
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Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism.

机译：在需要呼吸代谢的条件下，通过Fet3p提供的铁增加会导致啤酒酵母的复制寿命延长。

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摘要

We have previously shown that copper supplementation extends the replicative life span of Saccharomyces cerevisiae when grown under conditions forcing cells to respire. We now show that copper's effect on life span is through Fet3p, a copper containing enzyme responsible for high affinity transport of iron into yeast cells. Life span extensions can also be obtained by supplementing the growth medium with 1mM ferric chloride. Extension by high iron levels is still dependent on the presence of Fet3p. Life span extension by iron or copper requires growth on media containing glycerol as the sole carbon source, which forces yeast to respire. Yeast grown on glucose containing media supplemented with iron show no extension of life span. The iron associated with cells grown in media supplemented with copper or iron is 1.4-1.8 times that of cells grown without copper or iron supplementation. As with copper supplementation, iron supplementation partially rescues the life span of superoxide dismutase mutants. Cells grown with copper supplementation display decreased production of superoxide as measured by dihydroethidium staining.
机译：先前我们已经表明，在强迫细胞呼吸的条件下生长时，补充铜可延长酿酒酵母的复制寿命。现在，我们证明铜对寿命的影响是通过Fet3p来实现的，Fet3p是一种含铜的酶，负责铁向酵母细胞的高亲和力转运。通过向生长培养基中补充1mM氯化铁也可以延长使用寿命。高铁水平的延伸仍然取决于Fet3p的存在。铁或铜延长寿命需要在含有甘油作为唯一碳源的培养基上生长，这迫使酵母重新呼吸。在含铁的葡萄糖培养基上生长的酵母没有延长寿命。与在补充有铜或铁的培养基中生长的细胞相关的铁是未补充铜或铁的细胞的1.4-1.8倍。与补充铜一样，补充铁可部分挽救超氧化物歧化酶突变体的寿命。通过二氢乙锭染色测量，补充铜的细胞生长降低了超氧化物的产生。

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