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首页> 外文期刊>Eye >Langerhans' cells in vernal keratoconjunctivitis express the costimulatory molecule B7-2 (CD86), but not B7-1 (CD80).
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Langerhans' cells in vernal keratoconjunctivitis express the costimulatory molecule B7-2 (CD86), but not B7-1 (CD80).

机译：春季角膜结膜炎中的朗格汉斯细胞表达共刺激分子B7-2（CD86），但不表达B7-1（CD80）。

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PURPOSE: Vernal keratoconjunctivitis (VKC) is associated with T-helper 2 (TH2)-like cell response and increased immunoglobulin (Ig) E production. Recent studies have suggested that interactions between costimulatory molecules B7 on antigen-presenting cells and CD28 on T cells are critical for successful antigen presentation and the development of the TH2 immune response. The objective of this study was to examine the expression of costimulatory molecules CD28, B7-1 (CD80) and B7-2 (CD86) in conjunctival biopsies from patients with active VKC and normal controls. METHODS: Conjunctival biopsy specimens from 15 subjects with active VKC, and 8 control subjects, were studied by immunohistochemical techniques using a panel of monoclonal and polyclonal antibodies directed against CD28, B7-1 and B7-2 molecules. The phenotype of inflammatory cells expressing costimulatory molecules was examined by sequential double immunohistochemistry. RESULTS: In the normal conjunctiva, B7-2 was expressed on a few mononuclear cells in the epithelium and substantia propria in 5 of 8 specimens. There was no immunoreactivity for CD28 or B7-1. In VKC specimens, few B7-1+ mononuclear cells were noted in the substantia propria in 7 of 15 specimens. B7-2 was expressed on mononuclear cells in the epithelium and substantia propria in all specimens. Compared with normal controls, VKC specimens showed significantly more mononuclear cells expressing B7-2 (30.5+/-14.1 vs 1.88+/-2.5; p < 0.001). In VKC specimens, the numbers of mononuclear cells expressing B7-2 were significantly higher than the numbers of mononuclear cells expressing B7-1 (30.5+/-14.1 vs 2.3+/-3.1; p < 0.001). CD28 was expressed on mononuclear cells in the epithelium and substantia propria in 14 specimens. Colocalisation studies revealed that the majority of mononuclear cells expressing B7-2 were CD1a+ Langerhans' cells, and that the mononuclear cells expressing CD28 were CD3+ T lymphocytes. CONCLUSIONS: B7-2 is more widely and prominently expressed by Langerhans' cells compared with B7-1. The interaction of B7-2 with CD28 may mediate the development of the TH2 immune response in VKC. Thus the manipulation of this pathway could be an important target for the development of future therapies in VKC.
机译：目的：春季角膜结膜炎（VKC）与T-helper 2（TH2）样细胞反应和免疫球蛋白（Ig）E产生增加有关。最近的研究表明，抗原呈递细胞上的共刺激分子B7与T细胞上的CD28之间的相互作用对于成功的抗原呈递和TH2免疫应答的发展至关重要。这项研究的目的是检查在活动性VKC和正常对照的结膜活检中共刺激分子CD28，B7-1（CD80）和B7-2（CD86）的表达。方法：通过免疫组化技术，使用针对CD28，B7-1和B7-2分子的单克隆和多克隆抗体，研究了来自15位活动性VKC受试者和8位对照受试者的结膜活检标本。通过顺序双重免疫组织化学检查表达共刺激分子的炎性细胞的表型。结果：在正常结膜中，在8个样本中的5个中，B7-2在上皮和固有层中的几个单核细胞上表达。没有针对CD28或B7-1的免疫反应性。在VKC标本中，在15个标本中的7个中，固有层中几乎没有发现B7-1 +单核细胞。在所有标本中，B7-2在上皮和固有层的单核细胞上表达。与正常对照相比，VKC标本显示表达B7-2的单核细胞明显更多（30.5 +/- 14.1对1.88 +/- 2.5; p <0.001）。在VKC标本中，表达B7-2的单核细胞数量明显高于表达B7-1的单核细胞数量（30.5 +/- 14.1对2.3 +/- 3.1; p <0.001）。 CD28在14个标本中的上皮和固有层的单核细胞上表达。共定位研究表明，表达B7-2的单核细胞大多数是CD1a + Langerhans'细胞，表达CD28的单核细胞是CD3 + T淋巴细胞。结论：与B7-1相比，朗格汉斯细胞更广泛地表达B7-2。 B7-2与CD28的相互作用可能介导VKC中TH2免疫应答的发展。因此，对该途径的操纵可能是VKC中未来疗法发展的重要目标。

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