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首页> 外文期刊>Eye & contact lens >Strategies to optimize conditions for testing multipurpose contact lens solution efficacy against acanthamoeba
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Strategies to optimize conditions for testing multipurpose contact lens solution efficacy against acanthamoeba

机译：优化条件以测试多功能隐形眼镜溶液对棘阿米巴的功效的策略

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OBJECTIVES:: To optimize the growth, culture, and life cycle conditions for testing multipurpose solutions (MPS) against Acanthamoebatrophozoites and cysts to better inform the development of an appropriate test protocol. METHODS:: Two strains of Acanthamoeba castellanii were grown using 2 different methods, bacterized and axenic. Amoebae grown from both methods and from both strains were treated with 4 different MPS as trophozoites and cysts, which were generated using 4 encystment methods. Experiments were run in triplicate with controls. A 5-tube most probable number method was used to enumerate the survivors and to determine the log kills. Statistical analysis was performed using effect screening. RESULTS:: There was a marked difference in effectiveness among solutions, which varied with growth conditions (P<0.0001) and encystment method. Growth medium affected survival. In addition, there was a significant difference in cyst survival, which was dependent on encystment method (P=0.0013). The strain used was less of a factor in trophozoite resistance to MPS. Cyst resistance to MPS varied depending on which strain was used, but it was not a significant factor. CONCLUSIONS:: When designing a contact lens solution efficacy testing protocol for Acanthamoeba, care should be taken to control for variables that may distort results. An appropriate protocol should include growing Acanthamoeba bacterized and allowing them to encyst naturally. By choosing optimized testing conditions, a more realistic efficacy of contact lens solutions can be determined which will result in better and safer products on the market.
机译：目的：为了优化生长，培养和生命周期条件，以测试针对棘阿米巴滋虫和囊肿的多用途溶液（MPS），以更好地指导适当测试方案的开发。方法：使用两种不同的方法，分别通过细菌培养和无菌培养两种棘孢棘阿米巴菌株。将两种方法和两种菌株产生的变形虫都用4种不同的MPS处理，作为滋养体和囊肿，使用4种包囊方法生成。实验与对照一式三份进行。使用5管最有可能的数字方法来枚举幸存者并确定对数杀伤力。使用效果筛选进行统计分析。结果：溶液之间的有效性存在显着差异，其随生长条件（P <0.0001）和包囊方法而变化。生长培养基影响生存。此外，囊肿存活率存在显着差异，这取决于囊肿方法（P = 0.0013）。所使用的菌株较少是滋养体对MPS的抗性因素。囊肿对MPS的抗性随所使用的菌株而异，但这不是重要因素。结论：在设计用于棘阿米巴的隐形眼镜溶液功效测试方案时，应注意控制可能会使结果失真的变量。适当的规程应包括培养经过抗菌处理的棘阿米巴，并使其自然包裹。通过选择优化的测试条件，可以确定隐形眼镜解决方案的更实际的功效，从而可以生产出更好，更安全的市场产品。

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