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首页> 外文期刊>Eye & contact lens >Potential resistant morphotypes of acanthamoeba castellanii expressed in multipurpose contact lens disinfection systems
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Potential resistant morphotypes of acanthamoeba castellanii expressed in multipurpose contact lens disinfection systems

机译：多用途隐形眼镜消毒系统中表达的棘皮棘阿米巴虫的潜在抗性型

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OBJECTIVES:: The free-living amoeba Acanthamoeba castellanii is a rare cause of contact lens-associated microbial keratitis. The cyst stage of this amoeba is noted for its resistance to disinfection by multipurpose contact lens solutions (MPS). This report examines and reviews the potential survival modes of A. castellanii in MPS. METHODS:: Trophozoites of A. castellanii (ATCC 30234) at densities from 10 to near 10 were incubated in 3 different MPS in a laminar flow hood for 24 hours at ambient temperatures. The dried films of MPS and phosphate-buffered saline (PBS) controls were examined before and after the addition of a peptone-yeast extract-glucose recovery broth (PYG) for the presence of amoeboid trophozoites and resistance stages over at least 7 days. The parallel exposure of trophozoites to MPS without evaporation or addition of PYG was similarly examined. RESULTS:: Amoeboid trophozoites were not recovered in PYG nor were cyst-like structures observed in any MPS with near 10 densities. Progressively with 10 to 10 trophozoites, varied survival modes, particularly aggregates of trophozoites associated with cyst-like structures and occasional amoeboid forms and double-walled cysts with ostioles, became more evident. These morphotypes were most prominent after evaporation and typically first observed in the PYG. CONCLUSIONS:: Trophozoites of A. castellanii near 10 and progressively to 10 densities are capable of expressing a variety of "short-term" survival modes in MPS, notably with the added stress of evaporation. Expression of these alternate survival modes in MPS may relate, in part, to contamination of contact lens cases and difficulties in developing standardized MPS efficacy tests.
机译：目的：自由生存的变形虫变形虫是一种与隐形眼镜相关的细菌性角膜炎的罕见病因。该变形虫的囊肿阶段因其对多功能隐形眼镜溶液（MPS）的消毒能力而闻名。本报告审查并审查了MPS中的A. castellanii的潜在生存模式。方法：将密度为10至接近10的卡氏曲霉滋养体（ATCC 30234）在层流通风橱中的3种不同MPS中于环境温度下孵育24小时。在添加蛋白ept-酵母提取物-葡萄糖回收肉汤（PYG）之前和之后，检查MPS和磷酸盐缓冲盐水（PBS）对照的干膜，以检测是否存在变形虫滋养体和抗药性阶段，至少持续7天。类似地检查了滋养体平行暴露于MPS而不蒸发或不添加PYG的情况。结果：在PYG中未检出变形虫滋养体，在任何密度接近10的MPS中均未观察到囊样结构。逐渐使用10至10个滋养体，各种生存模式，尤其是与囊肿样结构和偶发性类阿波美体形式以及双耳囊壁囊肿相关的滋养体聚集体，变得更加明显。这些形态型在蒸发后最突出，通常首先在PYG中观察到。结论：A. castellanii的滋养体在密度接近10并逐渐提高到10时能够表达MPS中的各种“短期”生存模式，尤其是在增加蒸发压力的情况下。这些替代生存模式在MPS中的表达可能部分与隐形眼镜盒的污染以及制定标准化MPS功效测试的难度有关。

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