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Effect of a warming device on contact lens case contamination

机译：加热装置对隐形眼镜盒污染的影响

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PURPOSE:: To examine the effectiveness of heating contact lens cases after disinfection on reducing microbial contamination. METHODS:: One strain each of Pseudomonas aeruginosa (071) and Staphylococcus aureus (31) were used to set up robust biofilms in polypropylene contact lens cases. The effect of dilutions (from 1:10 to 1:1000) of trypticase soy broth (TSB) in phosphate-buffered saline and incubation time (24 to 48h) on the ability of strains to from biofilms with high levels of bacteria were first examined. Then the effect of increasing the temperature of incubation (from 14°C to 60°C) of biofilms during drying was examined. In the final set of experiments, biofilms of strains were subjected to heating in a warming device set to deliver 60°C for 3 hours, and the effect of this temperature after disinfection with a multipurpose disinfecting solution (MPDS; containing polyquat and Aldox) was examined by culturing the number of viable bacterial cells remaining. RESULTS:: A dilution of 1:100 TSB for S. aureus 31 and 1:1000 TSB for P. aeruginosa 071 together with an incubation time of 24 hours gave high numbers of viable cells of these 2 strains adhered to the contact lens cases. Having established the biofilms of bacteria, heating these to 60°C for 3 hours resulted in significant reductions in the number of viable cells that could be cultured (1 log reduction for S. aureus 31, P=0.0003; 3.5 log reduction for P. aeruginosa 071, P=0.002). Exposing the biofilms of cells to a disinfection cycle (6h at ambient temperature) in the presence of the MPDS and air drying at ambient temperature resulted in 2441±1237 colony-forming units/lens well for S. aureus 31 and 7401±4374 colony-forming units/lens well for P. aeruginosa 071. Increasing the drying temperature to 60°C resulted in zero viable cells (i.e., a‰14log reduction) for either bacterial type. CONCLUSIONS:: Using a warming device for contact lens cases after a disinfection cycle with an MPDS during drying for 3 hours results in substantial kill of biofilms of P. aeruginosa and S. aureus that have been formed in the wells of the cases.
机译：目的：：检查消毒后加热隐形眼镜盒对减少微生物污染的有效性。方法：使用一株铜绿假单胞菌（071）和金黄色葡萄球菌（31）在聚丙烯隐形眼镜盒中建立坚固的生物膜。首先检查了磷酸缓冲盐溶液中胰蛋白酶酶大豆肉汤（TSB）的稀释度（1:10至1：1000）和温育时间（24至48h）对菌株从高细菌水平的生物膜中分离的能力的影响。然后研究了在干燥过程中提高生物膜孵育温度（从14°C到60°C）的效果。在最后一组实验中，菌株的生物膜在设定​​为60°C的加热装置中加热3小时，用多功能消毒液（MPDS;含有多季铵盐和Aldox）消毒后，该温度的影响为通过培养剩余存活细菌细胞的数量进行检查。结果：金黄色葡萄球菌31的1：100 TSB稀释和铜绿假单胞菌071的1：1000 TSB稀释，以及24小时的孵育时间，使这2个菌株的大量活细胞粘附在隐形眼镜盒上。建立细菌的生物膜后，将其加热至60°C 3小时会导致可培养的活细胞数量显着减少（金黄色葡萄球菌31减少1 log，P = 0.0003; P。3.5减少log。铜绿071，P = 0.002）。在MPDS存在下，将细胞的生物膜暴露于消毒周期（在室温下6h）并在室温下空气干燥会导致金黄色葡萄球菌31和7401±4374菌落形成2441±1237个菌落形成单位/透镜孔。形成铜绿假单胞菌071的单位/透镜孔。将干燥温度提高到60°C，导致两种细菌的​​活细胞为零（即减少14log）。结论：在干燥3小时后用MPDS进行消毒循环后，对隐形眼镜盒使用加热装置会导致大量杀灭在盒孔中形成的铜绿假单胞菌和金黄色葡萄球菌的生物膜。

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