• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Identification and expression analysis of a new glycoside hydrolase family 55 exo-β-1,3-glucanase-encoding gene in Volvariella volvacea suggests a role in fruiting body development
【24h】

Identification and expression analysis of a new glycoside hydrolase family 55 exo-β-1,3-glucanase-encoding gene in Volvariella volvacea suggests a role in fruiting body development

机译：Volvolvella volvacea中新的糖苷水解酶家族55exo-β-1,3-葡聚糖酶编码基因的鉴定和表达分析表明其在子实体发育中起作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The edible straw mushroom Volvariella volvacea is an important crop in South East Asia and is predominantly harvested in the egg stage. Rapid stipe elongation and cap expansion result in a swift transition from the egg to elongation and maturation stage, which are subjected to fast senescence and deterioration. In other mushrooms, β-1,3-glucanases have been associated with degradation (softening) of the cell wall during stipe elongation and senescence. We present a new glycoside hydrolase family 55 (GH55) exo-β-1,3-glucanase gene, exg2, and highly conserved deduced EXG2 protein. The 3D model and presumed catalytic residues of V. volvacea EXG2 are identical to Lentinula edodes EXG2 and Phanerochaete chrysosporium Lam55A, supporting similar enzymatic functions. In addition to previous association to stipe elongation and senescence, our data clearly indicates a role for cap (pileus) expansion. Digital gene expression, quantitative PCR and isobaric tags for relative and absolute quantification analysis showed low exg2 and EXG2 levels in primordia, button, egg and elongation stages and significantly increased levels in the maturation stage. Subsequent relative quantitative PCR analysis designated expression of exg2 to the stipe in the elongation stage and to the pileus and stipe in the maturation stage. EXG2 cell wall softening activity, close correlation of exg2 expression with the principal expanding mushroom tissues and a strong conservation of expression patterns and protein sequences in other mushrooms, make V. volvacea exg2 an important candidate for future studies on mechanisms of fruiting body expansion and senescence causing commodity value loss.
机译：可食用的草菇草菇Volaceella volvacea是东南亚的重要农作物，主要在卵期收获。快速的刀柄伸长和盖扩展导致从卵到伸长和成熟阶段的快速转变，其经历快速的衰老和变质。在其他蘑菇中，β-1,3-葡聚糖酶与菌柄伸长和衰老过程中细胞壁的降解（软化）有关。我们提出了一个新的糖苷水解酶家族55（GH55）exo-β-1,3-葡聚糖酶基因，exg2，和高度保守的推导的EXG2蛋白。肠球菌EXG2的3D模型和推测的催化残基与香菇EXG2和Phanerochaete chrysosporium Lam55A相同，支持相似的酶功能。除了以前与菌柄伸长和衰老相关外，我们的数据清楚地表明了帽（绒毛）扩张的作用。用于相对和绝对定量分析的数字基因表达，定量PCR和同量异位标记显示，原基，纽扣，卵和延伸阶段的exg2和EXG2水平较低，而在成熟阶段的水平显着增加。随后的相对定量PCR分析将exg2的表达指定为伸长阶段的柄，成熟阶段为桩和柄的表达。 EXG2细胞壁软化活性，exg2表达与主要扩张的蘑菇组织密切相关以及其他蘑菇中表达模式和蛋白质序列的强烈保守性，使V. volvacea exg2成为未来子实体膨胀和衰老机制的重要候选造成商品价值损失。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号