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首页> 外文期刊>Biochemistry >Pigment assignment in the absorption spectrum of the photosystem II reaction center by site-selection fluorescence spectroscopy
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Pigment assignment in the absorption spectrum of the photosystem II reaction center by site-selection fluorescence spectroscopy

机译：位置选择荧光光谱法在光系统II反应中心吸收光谱中的颜料分配

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摘要

The steady state fluorescence properties of the photosystem II reaction center (D1-D2-cyt-b559 complex, PSII-RC) have been investigated by site-selection spectroscopy. The pattern of the vibronic bands in the emission spectra is used to identify the fluorescing species that have their absorption maxima on the red edge of the spectrum (at around 682 nm). At 10 K, even samples with a low content of red absorbing chlorophyll a (Chl) show pure Chl emission upon excitation at 685 nm, whereas at 77 K the fluorescence of the PSII-RCs is contributed to by Chl and pheophytin a (Pheo) in a ratio of roughly 8:2. These results allow an unequivocal distinction between two different spectral decompositions that were recently suggested for the absorption spectrum of the PSII-RC [Konermann, L., & Holzwarth, A. R. (1996) Biochemistry 35, 829]. Only one of these decompositions is compatible with the experimental data presented here according to which the absorption on the red edge of the spectrum is dominated by an accessory Chl.
机译：已通过位点选择光谱研究了光系统II反应中心（D1-D2-cyt-b559配合物，PSII-RC）的稳态荧光性质。发射光谱中的电子振动带谱用于识别其吸收最大值位于光谱红色边缘（约682 nm）的发荧光物质。在10 K时，即使在低含量的吸收红叶绿素a（Chl）的样品中，在685 nm激发时也显示纯Chl发射，而在77 K时，PSII-RCs的荧光由Chl和脱镁叶绿素a（Pheo）促成以大约8：2的比例。这些结果可以明确区分两个最近对PSII-RC的吸收光谱提出的光谱分解[Konermann，L.，＆Holzwarth，A. R.（1996）Biochemistry 35，829]。这些分解中只有一个与此处提供的实验数据兼容，根据该数据，在光谱红色边缘的吸收主要由辅助Ch1决定。

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