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首页> 外文期刊>General and comparative endocrinology >In ovo treatment with an estrogen receptor alpha selective agonist causes precocious development of the female reproductive tract of the American alligator (Alligator mississippiensis)
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In ovo treatment with an estrogen receptor alpha selective agonist causes precocious development of the female reproductive tract of the American alligator (Alligator mississippiensis)

机译：在用雌激素受体的卵内治疗中，α选择性激动剂引起美洲短吻鳄（Alligator mississippiensis）雌性生殖道的早熟发育。

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The molecular signaling processes involved the differentiation of the Mtillerian duct (MD) into the female reproductive tract, or oviduct, in non-mammalian vertebrates are not well understood. Studies in mammals and birds indicate that steroid hormones play a role in this process, as the embryonic MD has been shown to be vulnerable to exogenous estrogens and progestins and environmental endocrine disrupting contaminants. In a previous study, developmental treatment with an estrogen receptor alpha (ER alpha) agonist, 4,4',4"(4-propyl-[1H]-pyrazole-1,3,5-triy1)trisphenol (PPT), induced significant enlargement of the MD in alligator embryos incubated at a male-producing temperature, which was not observed in embryos treated with an estrogen receptor beta (ER beta) agonist, 7-bromo-2-(4-hydroxyphenyl)-1,3-benzoxazol-5-ol (WAY 200070), or with 17 beta-estradiol (E-2). In order to understand the role of estrogen signaling in female alligator oviduct development, we incubated eggs at a female-producing temperature and treated them with E-2 and these ER selective agonists, PPT and WAY 200070, just prior to the thermosensitive window of sex determination. At stage 27, one stage prior to hatching, PPT induced significant enlargement of the MD with precocious development of secretory glands and connective tissue differentiation similar to characteristics of mature adult oviduct. PPT treatment in ovo increased mRNA expression of ER beta, progesterone receptor, androgen receptor and insulin-like growth factor 1 in MD at stage 27, while expression of ERa was decreased. Neither WAY 200070 nor E-2 treatment induced these effects seen in PPT-treated MD. The results of this study provide insight into the critical factors for healthy reproductive system formation in this sentinel species, although further investigation is needed to determine whether the observed phenomena are directly due to selective stimulation of ER alpha or related to some other aspect of PPT treatment. (C) 2016 Elsevier Inc. All rights reserved.
机译：在非哺乳动物脊椎动物中，涉及Mtillerian导管（MD）分化为雌性生殖道或输卵管的分子信号传导过程尚不清楚。对哺乳动物和鸟类的研究表明，类固醇激素在此过程中起作用，因为已证明胚胎MD易受外源雌激素和孕激素以及破坏环境内分泌的污染物的影响。在以前的研究中，诱导了雌激素受体α（ER alpha）激动剂4,4'，4“（4-丙基-[1H]-吡唑-1,3,5-triy1）三酚（PPT）的发育性治疗在雄性产卵温度下孵育的扬子鳄胚胎中MD的显着增大，而在用雌激素受体β（ERβ）激动剂7-溴-2-（4-羟苯基）-1,3-处理的胚胎中未观察到苯并恶唑-5-醇（WAY 200070）或与17β-雌二醇（E-2）结合为了了解雌激素信号在雌性短吻鳄输卵管发育中的作用，我们在雌性产卵温度下孵育卵，并对其进行了处理。 E-2和这些ER选择性激动剂PPT和WAY 200070，就在性别确定的热敏窗之前。在第27阶段，即孵化之前的一个阶段，PPT导致MD显着增大，分泌腺和结缔组织过早发育分化类似于成熟的成年输卵管特征。卵内PPT治疗增加mRN 27岁时MD中ERβ，孕激素受体，雄激素受体和胰岛素样生长因子1的表达降低，而ERa的表达降低。在PPT处理的MD中，WAY 200070和E-2处理均未引起这些作用。这项研究的结果为深入了解该前哨物种中健康生殖系统形成的关键因素提供了见识，尽管还需要进一步研究以确定观察到的现象是否直接由于选择性地刺激ER alpha或与PPT治疗的其他方面有关。 （C）2016 Elsevier Inc.保留所有权利。

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