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Nippostrongylus brasiliensis: increase of sialomucins reacting with anti-mucin monoclonal antibody HCM31 in rat small intestinal mucosa with primary infection and reinfection.

机译:巴西夜蛾:在初次感染和再感染的大鼠小肠粘膜中,唾液铝蛋白与抗粘蛋白单克隆抗体HCM31反应的增加。

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摘要

Infections with the parasitic helminth, Nippostrongylus brasiliensis, cause changes in rat small intestinal goblet cell mucin, particularly in the peripheral sugar residues of oligosaccharide. These changes may correlate with expulsion. In this study, we examined changes in mucin oligosaccharides caused by primary infection and reinfection with N. brasiliensis, using two monoclonal antibodies, HCM31 and PGM34, that react with sialomucin and sulfomucin, respectively. Enzyme-linked immunosorbent assay of jejunal mucins showed that the relative reactivity of mucins with HCM31, but not PGM34, increased up to 16 days after primary infection and 6 days after reinfection, the times when the worms were expelled from the rats. Immunohistochemical studies confirmed that goblet cells stained with HCM31 greatly increased at the time of worm expulsion. These results indicate that the marked increase observed in HCM31-reactive sialomucins may be related to expulsion of the worms.
机译:巴西寄生虫(Nippostrongylus brasiliensis)感染后,会引起大鼠小肠杯状细胞黏蛋白的改变,尤其是低聚糖的外周糖残基。这些变化可能与驱逐有关。在这项研究中,我们使用两种单克隆抗体HCM31和PGM34分别与唾液白蛋白和磺胺嘧啶起反应,研究了巴西原虫的初次感染和再感染引起的粘蛋白寡糖变化。空肠粘蛋白的酶联免疫吸附试验表明,粘蛋白与HCM31而不是PGM34的相对反应性在初次感染后长达16天和再感染后6天(从蠕虫被驱逐出鼠的时间)开始增加。免疫组织化学研究证实,驱逐蠕虫时,用HCM31染色的杯状细胞大大增加。这些结果表明,在HCM31反应性唾液铝蛋白中观察到的明显增加可能与蠕虫的排出有关。

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