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首页> 外文期刊>Bulletin of the Korean Chemical Society >Chemical Imaging Analysis of the Micropatterns of Proteins and Cells Using Cluster Ion Beam-based Time-of-Flight Secondary Ion Mass Spectrometry and Principal Component Analysis
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Chemical Imaging Analysis of the Micropatterns of Proteins and Cells Using Cluster Ion Beam-based Time-of-Flight Secondary Ion Mass Spectrometry and Principal Component Analysis

机译：基于簇离子束的飞行时间二次离子质谱和主成分分析的蛋白质和细胞微模式的化学成像分析

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Micropatterns of streptavidin and human epidermal carcinoma A431 cells were successfully imaged, as received and without any labeling, using cluster Au3~+ ion beam-based time-of-flight secondary ion mass spectrometry (TOF-SIMS) together with a principal component analysis (PCA). Three different analysis ion beams (Ga~+, Au~+ and Au3~+) were compared to obtain label-free TOF-SIMS chemical images of micropatterns of streptavidin, which were subsequently used for generating cell patterns. The image of the total positive ions obtained by the Au3~+ primary ion beam corresponded to the actual image of micropatterns of streptavidin, whereas the total positive-ion images by Ga~+ or Au~+ primary ion beams did not. A PCA of the TOF-SIMS spectra was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from the raw data and used in the second PCA run, which resulted in a contrasted - and corrected - image of the micropatterns of streptavidin by the Ga~+ and Au~+ ion beams. The findings herein suggest that using cluster-ion analysis beams and multivariate data analysis for TOF-SIMS chemical imaging would be an effectual method for producing label-free chemical images of micropatterns of biomolecules, including proteins and cells.
机译：使用基于簇Au3〜+离子束的飞行时间二次离子质谱（TOF-SIMS）结合主成分分析，成功地对链霉亲和素和人表皮癌A431细胞的微模式进行了成像，且未进行任何标记。 PCA）。比较了三种不同的分析离子束（Ga〜+，Au〜+和Au3〜+），以获得链霉亲和素微模式的无标记TOF-SIMS化学图像，随后将其用于生成细胞模式。 Au3〜+一次离子束获得的总正离子图像与链霉亲和素的微图案的实际图像相对应，而Ga〜+或Au〜+一次离子束获得的总正离子图像却不相同。最初进行了TOF-SIMS光谱的PCA，以鉴定链霉亲和素的特征性次级离子。从原始数据重建每个特征离子的化学图像，并将其用于第二次PCA运行，从而通过Ga〜+和Au〜+离子束对链霉亲和素的微图案进行了对比和校正后的图像。本文的发现表明，使用簇离子分析束和多变量数据分析进行TOF-SIMS化学成像将是一种有效的方法，用于产生包括蛋白质和细胞在内的生物分子微模式的无标记化学图像。

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