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首页> 外文期刊>Genetic Resources and Crop Evolution >Identification of SUB1A alleles from wild rice Oryza rufipogon Griff.
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Identification of SUB1A alleles from wild rice Oryza rufipogon Griff.

机译：从野生稻米红麦芽中鉴定出SUB1A等位基因。

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摘要

Submergence stress is a major constraint to rice production in South and Southeast Asia. Most rice (Oryza sativa L.) cultivars die within a week of complete submergence, while a small number of accessions are submergence-tolerant for up to 2 weeks or more. These cultivars have the tolerant allele of the SUB1A gene, one of three ERF genes at this locus on rice chromosome 9. In all O. sativa varieties studied, the SUB1A gene is limited to a subset of indica accessions of O. sativa. Thus far, there has been no published report of the SUB1A gene in wild rice species. Here we report evidence of the SUB1A gene found in wild species of O. rufipogon Griff. accessions by the use of degenerate primers corresponding to the most highly conserved regions of the SUB1 locus. The results indicated that two SUB1A-like alleles, e. g. OrSub1A-1 and OrSub1A-2, were identified from two O. rufipogon accessions. Submergence treatment shows that both of the accessions with SUB1A-like genes were submergence-intolerant. This preliminary study provides insight into the origin and allelic variation of SUB1A, an agronomically important gene that is rapidly being introduced into widely-grown rice cultivars.
机译：淹没压力是南亚和东南亚水稻生产的主要制约因素。大多数水稻（Oryza sativa L.）品种在完全浸没后一周内死亡，而少数品种对浸没的耐受性长达2周或更长时间。这些品种具有水稻1号染色体上该基因座的SUB1A基因的耐性等位基因，这是三个ERF基因之一。在所有研究的水稻品种中，SUB1A基因仅限于水稻品种的一部分。迄今为止，还没有公开的关于野生稻种中SUB1A基因的报道。在这里，我们报告在rufipogon Griff野生物种中发现SUB1A基因的证据。通过使用与SUB1基因座最保守的区域相对应的简并引物来保藏。结果表明有两个SUB1A样等位基因，例如。 G。 OrSub1A-1和OrSub1A-2，是从两个O. rufipogon品种中鉴定出来的。淹没处理表明，两个带有SUB1A样基因的种属均具有淹没耐受性。这项初步研究提供了对SUB1A的起源和等位基因变异的了解，SUB1A是一种农业重要基因，已被迅速引入广泛种植的水稻品种中。

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