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首页> 外文期刊>Genetic Resources and Crop Evolution >Morphological and molecular diversity of an underutilized fruit crop - Cordia myxa L. germplasm from the arid region of Rajasthan, India
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Morphological and molecular diversity of an underutilized fruit crop - Cordia myxa L. germplasm from the arid region of Rajasthan, India

机译:未充分利用的水果作物-印度拉贾斯坦邦干旱地区的Cordia myxa L.种质的形态和分子多样性

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Twenty two germplasm accessions of Cordia myxa were collected from Rajasthan and established at the field gene bank for conservation and evaluation. Morphological characterization of 10 year-old trees for 17 traits indicated wide variations among the accessions tested. Higher number of flowers per cyme was found in accession ACHM11 and higher pulp:stone ratio in AHCM25. Overall, AHCM22 was found to be a superior germplasm line for most of the horticulturally useful traits among the accessions tested as it had higher percent of fruit set, pulp:stone ratio and fruit weight. High significant positive correlation was obtained between leaf, fruit characters and pulp:stone ratio. However, these characters were found to be negatively correlated with number of flowers per cyme. Out of 50 random decamer primers used for random amplification (RAPD), 25 were polymorphic. Average polymorphism resolved by these markers among these accessions was 69.8% with an average polymorphic information content of 0.43. Genetic diversity revealed by Jaccard's co-efficient was between 0.44 and 0.94, and three major clusters were identified among these accessions by phylogenetic analysis using NTSYSpc-2.02e software. RAPD markers associated with leaf size and pulp:stone ratio were also identified. This study shows the existence of high genetic diversity among these accessions.
机译:从拉贾斯坦邦收集了22个Cordia myxa种质种质,并将其建立在田间基因库中,以进行保存和评估。 10年生树木具有17个性状的形态特征表明,测试的种质之间差异很大。在登录号ACHM11中发现了每百里香的花数更高,而在AHCM25中发现了更高的纸浆/石比率。总体而言,在测试的种质中,AHCM22是大多数园艺有用性状的优良种系,因为它具有较高的坐果率,果肉:结石比和果实重量。叶片,果实性状和果肉:石头比率之间具有高度显着的正相关。但是,发现这些特征与每百里香的花数呈负相关。在用于随机扩增(RAPD)的50个随机十聚体引物中,有25个是多态性的。这些标记物中这些标记解析的平均多态性为69.8%,平均多态性信息含量为0.43。 Jaccard系数揭示的遗传多样性在0.44至0.94之间,并使用NTSYSpc-2.02e软件通过系统发育分析在这些种质中鉴定出三个主要簇。还确定了与叶大小和纸浆:石材比率相关的RAPD标记。这项研究表明这些种之间存在高度遗传多样性。

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