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Single seed descent: a tool to exploit durum wheat (Triticum durum Desf.) genetic resources

机译:单种子后代:利用硬质小麦(Triticum durum Desf。)遗传资源的工具

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A large wealth of genetic diversity is present in the diversity centers of durum wheat. However, modern breeding selection methods are mostly based on improvement of ,lite lines which have a narrow genetic base; this limits a lot the genetic pool which breeders can exploit for the production of new varieties ready to face the predicted climate changes or adaptation to new cultivation areas. The oncoming changing climatic conditions claim for the need of finding new adaptation traits to both abiotic and biotic stresses to be introduced in modern ,lite varieties. These traits are often found in landraces stored in germplasm collections. The Eurisco database reports a total of over 17,000 durum accessions, some 6,800 of which are traditional varieties and/or landraces: Besides their incontestable usefulness, their great number hampers the possibility to explore their genepool. A different approach to reduce the number of genotypes to screen for new useful traits to use in breeding programs while maintaining a good representation of the genetic background, is the constitution of a working durum wheat collection selected by mean of a single seed descent (SSD) approach so far called SSD genotypes. The working collection, accounts for over approximately 450 genotypes representative of 40 countries mainly concentrated in the Mediterranean area and Horn of Africa. The SSD genotypes were subjected to large scale SNP screening by ILLUMINA Infinium iSelect 90k wheat chip and the data were analysed to assess the heterozygosity and the genetic diversity present in the SSD population. Moreover the population was preliminary phenotyped following field growth. Both analyses give the picture of a good level of genetic variability represented in the SSD genotypes. This makes this collection a good candidate for a new "genetool" for durum wheat breeding and research.
机译:硬质小麦的多样性中心存在大量的遗传多样性。然而,现代育种选择方法主要是基于改良具有较窄遗传基础的优良品系。这在很大程度上限制了育种者可以利用这些遗传库来生产新品种,以准备应对预期的气候变化或适应新的耕种地区。即将到来的不断变化的气候条件要求寻找新的适应性特征,以适应现代优良品种引入的非生物和生物胁迫。这些性状通常在种质收集物中存储的地方品种中发现。 Eurisco数据库总共报告了17,000余种硬质杜伦登录品,其中6,800是传统品种和/或地方品种:除了其无可争议的实用性外,数量众多还阻碍了探索其种质库的可能性。减少基因型数量以筛选新的有用性状以用于育种计划,同时保持良好的遗传背景表现的另一种方法是,通过单一种子后代(SSD)选择工作的硬质小麦品种的构成迄今为止,这种方法被称为SSD基因型。该工作集涵盖了40个国家的大约450种基因型,这些基因型主要集中在地中海地区和非洲之角。通过ILLUMINA Infinium iSelect 90k小麦芯片对SSD基因型进行了大规模SNP筛选,并对数据进行了分析,以评估SSD群体中存在的杂合性和遗传多样性。此外,种群在田间生长后被初步表型化。两种分析都给出了以SSD基因型代表的遗传变异水平较高的图景。这使得该系列非常适合用于硬粒小麦育种和研究的新“ genetool”。

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