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Diversity of Oxalis tuberosa Molina: a comparison between AFLP and microsatellite markers

机译：alis草Molina的多样性：AFLP和微卫星标记之间的比较

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摘要

Traditional crops contribute to food security and agroecological sustainability, but their diversity is increasingly threatened by complex interplays of local and global sociocultural and economic change. Molecular markers are powerful tools to measure and characterize this diversity, and comparisons among different molecular marker systems are necessary to assess their appropriateness in different research contexts. Using a common sample set, we compare amplified fragment length polymorphism (AFLP) and microsatellite (simple sequence repeats; SSRs) techniques to assess their utility in research on the Andean tuber crop oca (Oxalis tuberosa Molina, Oxalidaceae). We find that 26 of 27 individuals have distinct AFLP genotypes, and all 27 individuals have distinct SSR genotypes. Both markers systems cluster samples in agreement with morphotype groups and separate clusters with similar strength, but more variation occurs within AFLP-based clusters than within SSR-based clusters. In addition, correlation between marker systems of pairwise distances is positive and significant (R = 0.831, p = 0.001). Ultimately, we discuss each system's advantages and disadvantages for future oca diversity research.
机译：传统作物有助于粮食安全和农业生态可持续性，但其多样性正日益受到当地和全球社会文化与经济变化复杂相互作用的威胁。分子标记是测量和表征这种多样性的有力工具，不同分子标记系统之间的比较对于评估它们在不同研究背景下的适用性是必要的。使用一个常见的样本集，我们比较了扩增片段长度多态性（AFLP）和微卫星（简单序列重复; SSR）技术，以评估其在安第斯块茎作物oca（Oxalis tuberosa Molina，Oxalidaceae）研究中的效用。我们发现27个人中有26个人具有不同的AFLP基因型，所有27个人具有不同的SSR基因型。两种标记系统均以与形态型群一致的方式对样品进行聚类，并以相似的强度对单独的聚类进行聚类，但是在基于AFLP的聚类中比在基于SSR的聚类中发生更多的变异。另外，成对距离的标记系统之间的相关为正且显着（R = 0.831，p = 0.001）。最终，我们讨论了每种系统在将来进行oca多样性研究中的优缺点。

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