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Q gene variability in wheat species with different spike morphology

机译:不同穗形形态小麦品种Q基因变异性

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摘要

Q gene is the major domestication gene of wheat and many questions concerning Q gene genetics, including Q gene variability and its functional influence on phenotype, remain unanswered for the majority of wheat species. Here we crossed wheat species with dominant (Q) and recessive (q) alleles and confirmed that Q gene controls threshability, rachis fragility and spike shape traits. In the present study 18 new Q gene sequences were obtained and the Q gene sequences from 42 di- and polyploid wheat species with variable spike morphology were analyzed. We identified correlation between Q gene variability (coding mutation 329Val/Ile, promoter variability, microRNA172 binding site substitution) and threshability, rachis fragility and spike shape traits in polyploid wheat species. The analysis of 3D structures of q and Q proteins indicated that 329Val/Ile mutation does not affect overall protein structure and likely protein activity. We conclude that alterations in all three regions are essential for the formation of free-threshing non fragile normal phenotype in polyploid wheat.
机译:Q基因是小麦的主要驯化基因,大多数小麦品种仍未回答有关Q基因遗传学的许多问题,包括Q基因变异性及其对表型的功能影响。在这里,我们将小麦等位基因与优势(Q)和隐性(q)等位基因杂交,并证实了Q基因控制着脱粒性,轮轴脆性和穗形特征。在本研究中,获得了18个新的Q基因序列,并分析了42种具有不同穗形形态的二倍体和多倍体小麦品种的Q基因序列。我们确定了多倍体小麦品种中Q基因变异性(编码突变329Val / Ile,启动子变异性,microRNA172结合位点置换)与脱粒性,Rachis脆性和穗状性状之间的相关性。 q和Q蛋白质的3D结构分析表明329Val / Ile突变不会影响整体蛋白质结构和可能的蛋白质活性。我们得出结论,这三个区域的变化对于多倍体小麦中自由脱粒的非脆弱正常表型的形成至关重要。

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