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A study of genetic diversity in bottle gourd [Lagenaria siceraria (Molina) Standl.] population, and implication for the historical origins on bottle gourds in Turkey

机译:葫芦[Lagenaria siceraria(Molina)Standl。]种群遗传多样性的研究及其对土耳其葫芦历史渊源的启示

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Bottle gourd [Lagenaria siceraria (Molina) Standl.] is one of the oldest cultivated plants, native to Africa and it distributed to over the World. Its dispersal route from Africa to America and Asia is still subject of studies. Similarly, when and how bottle gourd entered into Turkey is not known. In previous studies, bottle gourd germplasm collecting activities were undertaken in the country and the germplasm were analyzed morphologically. Here we report molecular analysis of 60 Turkish bottle gourds along with 31 diverse exotic accessions. Eighth Simple Sequence Repeats and two chloroplast loci of 91 accessions were analyzed. Capillary electrophoresis was used for DNA size fragman separation. The number of alleles per locus ranged from 2 to 13, with a mean of 7. Mean values for expected heterozygosity, observed heterozgosity, and polymorphism information averaged 0.5, 0.13 and 0.50, respectively, thereby sugguesting very low (0.13) genetic diversity in a very diverse population. An unweighted pair group method with arithmetic mean dendrogram was constructed, Indian accessions clearly separated from the rest. Among the remaining samples, neither Turkish landraces grouped together based on their georgraphic origin, nor clear speration occurred according to origin continent. Interestingly, this co-dominant marker analysis shows close molecular allelic profile among bottle gourds originated from far different countries.
机译:[Lagenaria siceraria(Molina)Standl。]是最古老的栽培植物之一,原产于非洲,并分布于世界各地。它从非洲到美洲和亚洲的传播途径仍然是研究的主题。同样,葫芦何时以及如何进入土耳其尚不清楚。在以前的研究中,该国进行了葫芦种质的收集活动,并对其形态进行了分析。在这里,我们报告了60种土耳其Turkish的分子分析以及31种不同的外来种质。分析了第八个简单重复序列和91个登录号的两个叶绿体位点。毛细管电泳用于DNA大小弗格曼分离。每个基因座的等位基因数量范围为2到13,平均为7。预期杂合性,观察到的杂合性和多态性信息的平均值分别平均为0.5、0.13和0.50,因此建议在一个地方的遗传多样性非常低(0.13)。人口非常多样化。构造了具有算术平均树状图的非加权对群方法,印度种与其他种明显分开。在其余的样本中,既没有根据地理起源将土耳其地方种归为一类,也没有根据起源大陆发生明显的散乱。有趣的是,这种共同主导的标记分析显示了来自不同国家的葫芦中的分子等位基因分布紧密。

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