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首页> 外文期刊>Genetic Resources and Crop Evolution >Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers
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Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers

机译：使用ISSR，RAPD和SSR标记分析欧洲和亚洲无花果变种（Ficus carica L.）的遗传多样性

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Nineteen fig varieties and lines from Europe and Asia have been fingerprinted by ISSR, RAPD, and SSR markers, respectively, using 13, 19, and 13 primer combinations. All primers produced 258 loci, with the highest number of loci (119) generated by RAPD (R p: 48.42). Clustering analysis was applied to the three marker datasets to elucidate the genetic structure and relationships among these varieties. Mean genetic similarities were 0.787, 0.717, and 0.749, respectively, as determined using ISSR, RAPD, and SSR. Each marker system produced incompletely separated clusters, although a weak binding group based on race type appeared in the combined dataset. Comparisons of coefficients revealed no correlation between different similarity matrices; congruence was observed between similarity matrices and co-phenetic matrices in all markers. Analysis of molecular variance (AMOVA) showed that most of the total polymorphism was attributable to within-group variance (ISSRs + RAPDs, 97.41%; SSRs, 90.18%). These results suggest that the genetic diversity of this fig population is low and that multiple marker utilization is critical to estimate the relatedness of figs at the variety level. Additionally, it was presumed that 'Houraihi', the oldest variety in Japan, was disseminated independently of other foreign varieties in the 17th century or before then.
机译：使用13、19和13个引物组合分别通过ISSR，RAPD和SSR标记对来自欧洲和亚洲的19个无花果变种和品系进行了指纹识别。所有引物均产生258个基因座，其中RAPD产生的基因座数量最多（119）（R p：48.42）。将聚类分析应用于三个标记数据集，以阐明这些品种之间的遗传结构和关系。使用ISSR，RAPD和SSR确定的平均遗传相似性分别为0.787、0.717和0.749。每个标记系统产生不完全分离的簇，尽管基于种族类型的弱结合基团出现在组合数据集中。系数比较表明，不同相似度矩阵之间没有相关性。在所有标记中相似性矩阵和共价矩阵之间观察到一致。分子变异分析（AMOVA）显示，大多数总多态性可归因于组内变异（ISSRs + RAPDs，97.41％; SSRs，90.18％）。这些结果表明，该无花果种群的遗传多样性较低，多重标记的利用对于在品种水平上估计无花果的相关性至关重要。此外，据推测，日本最早的变种“ Houraihi”在17世纪或更早的时候独立于其他外国品种进行传播。

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