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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Components of the spindle assembly checkpoint regulate the anaphase-promoting complex during meiosis in Caenorhabditis elegans.
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Components of the spindle assembly checkpoint regulate the anaphase-promoting complex during meiosis in Caenorhabditis elegans.

机译：秀丽隐杆线虫减数分裂期间，纺锤体装配检查点的组件调节后期促进复合物。

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摘要

Temperature-sensitive mutations in subunits of the Caenorhabditis elegans anaphase-promoting complex (APC) arrest at metaphase of meiosis I at the restrictive temperature. Embryos depleted of the APC co-activator FZY-1 by RNAi also arrest at this stage. To identify regulators and potential substrates of the APC, we performed a genetic suppressor screen with a weak allele of the APC subunit MAT-3/CDC23/APC8, whose defects are specific to meiosis. Twenty-seven suppressors that resulted in embryonic viability and larval development at the restrictive temperature were isolated. We have identified the molecular lesions in 18 of these suppressors, which correspond to five genes. In addition to a single intragenic suppressor, we found mutations in the APC co-activator fzy-1 and in three spindle assembly checkpoint genes, mdf-1, mdf-2, and mdf-3/san-1, orthologs of Mad1, Mad2, and Mad3, respectively. Reduction-of-function alleles of mdf-2 and mdf-3 suppress APC mutants and exhibit pleiotropic phenotypes in an otherwise wild-type background. Analysis of a single separation-of-function allele of mdf-1 suggests that MDF-1 has a dual role during development. These studies provide evidence that components of the spindle assembly checkpoint may regulate the metaphase-to-anaphase transition in the absence of spindle damage during C. elegans meiosis.
机译：秀丽隐杆线虫后期促进复合物（APC）的亚基中的温度敏感突变在限制性温度下停在减数分裂I的中期。 RNAi耗尽了APC共激活因子FZY-1的胚胎也在此阶段停滞。为了鉴定APC的调控因子和潜在底物，我们对APC亚基MAT-3 / CDC23 / APC8的弱等位基因进行了遗传抑制子筛选，其缺陷特定于减数分裂。分离了二十七种抑制子，它们在限制温度下可导致胚胎存活和幼虫发育。我们已经鉴定出18种抑制物的分子损伤，它们与5个基因相对应。除了单个基因内抑制子外，我们还发现了APC共激活因子fzy-1和三个纺锤体装配检查点基因mdf-1，mdf-2和mdf-3 / san-1中的突变，Mad1，Mad2的直系同源基因，和Mad3分别。 mdf-2和mdf-3的功能降低等位基因抑制APC突变体并在其他野生型背景下表现出多效性表型。对mdf-1的单个功能分离等位基因进行分析表明，MDF-1在发育过程中具有双重作用。这些研究提供了证据，表明纺锤体减数分裂过程中纺锤体不存在纺锤体损害的情况下，纺锤体装配检查点的组件可调节中期到后期的过渡。

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