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首页> 外文期刊>Genetic Resources and Crop Evolution >Durum wheat (Triticum durum Desf.) Mediterranean landraces as sources of variability for allelic combinations at Glu-1/Glu-3 loci affecting gluten strength and pasta cooking quality
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Durum wheat (Triticum durum Desf.) Mediterranean landraces as sources of variability for allelic combinations at Glu-1/Glu-3 loci affecting gluten strength and pasta cooking quality

机译：硬质小麦（Triticum durum Desf。）地中海地方品种是Glu-1 / Glu-3位点等位基因组合变异的来源，影响面筋强度和面食烹饪质量

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With the aim of identifying durum wheat landraces (LR) with a potential use in breeding programs for gluten strength enhancement, the allelic combinations present at five glutenin loci were determined in a collection of 155 LR from 21 Mediterranean countries. A set of 18 modern cultivars (MC) was used for comparison. Gluten strength was determined by SDS-sedimentation test on grain samples from field experiments conducted during 3 years. A total number of 131 different allelic/banding pattern combinations were found. Taking together high (HMW-) and low (LMW-) molecular weight glutenin subunit loci resulted in 126 combinations in LR, but only nine in MC, which are characterized for having strong gluten. Two LMW-2 type models were identified in the collection and LMW-1 types were absent. LMW-2 was present in 78 % of MC, including the only three with outstanding gluten strength (Ocotillo, Claudio and Meridiano), while 14 % of the LR had LMW-2 and 6 % LMW-2(-). In the LR a known combination LMW-2 (aaa) and three new ones had a positive effect on the gluten strength. LMW-2 models were found in high frequency in LR from Italy and the three Maghreb countries; from medium to low frequencies in genotypes from Turkey, Jordan, Lebanon, Portugal and Spain, and were absent in the remaining countries. The large variability found in LR proved their potential value in breeding to broaden the genetic basis of gluten quality improvement. Genotypes interesting for breeding purposes are identified
机译：为了确定硬粒小麦地方品种（LR）在提高面筋强度的育种计划中的潜在用途，在来自21个地中海国家的155个LR的集合中确定了存在于5个谷蛋白基因座上的等位基因组合。一组18个现代品种（MC）用于比较。通过SDS沉降试验对3年间进行的田间试验的谷物样品测定面筋强度。共发现131种不同的等位基因/条带模式组合。结合高分子量（HMW-）和低分子量（LMW-）的谷蛋白亚基位点导致LR中有126个组合，而MC中只有9个组合，其特征是具有强筋蛋白。在该集合中确定了两个LMW-2类型模型，而缺少LMW-1类型。 LMW-2存在于78％的MC中，包括仅有的三个具有优异面筋强度的蛋白（Ocotillo，Claudio和Meridiano），而14％的LR具有LMW-2和6％的LMW-2（-）。在LR中，已知的LMW-2（aaa）和三个新的组合对面筋强度具有积极影响。在意大利和马格里布三个国家的LR中发现了LMW-2模型的高频部分。土耳其，约旦，黎巴嫩，葡萄牙和西班牙的基因型从中频到低频，其余国家则不存在。 LR中发现的巨大变异性证明了其在育种中的潜在价值，以拓宽面筋质量改良的遗传基础。确定育种目的基因型

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