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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic diversity of Swiss maize (Zea mays L. ssp. mays) assessed with individuals and bulks on agarose gels
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Genetic diversity of Swiss maize (Zea mays L. ssp. mays) assessed with individuals and bulks on agarose gels

机译：用琼脂糖凝胶上的个体和散装体评估瑞士玉米（Zea mays L. ssp。mays）的遗传多样性

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摘要

About 65 years ago, more than 150 Swiss maize landraces (Zea mays L. ssp. mays) of the flint type were collected and conserved ex situ. Due to the climatically and culturally diverse environment of the Alps, a considerable genetic diversity of this material was assumed. To prove this, an efficient method was required to carry out genetic profiling of all the accessions in the Swiss Gene Bank. Simple sequence repeat marker (SSR) profiling in combination with the visualization of the polymerase chain reaction (PCR) products on agarose gels was chosen. Here a set of 19 different landrace accessions was analyzed to: (i) investigate their genetic diversity, (ii) investigate and display the population structure and (iii) determine whether DNA bulks rather than single plants can be used for such analyses. Four repeated samples of one accession were found to be much closer to one another than to the rest of accessions. Furthermore, specific alleles were identified for several accessions. The PCR products of the bulked DNA samples represented only a small part of the variation revealed by the analysis of individuals. Loci with four base repeat motifs performed better in the analysis of bulks than loci with other repeat motifs. The correlation between genetic distance matrices, based on the analysis of individuals and bulks, respectively, was significant. Thus, the single plant approach allowed for sufficient differentiation of accessions, and DNA bulks visualized on agarose gels led to correlated genetic distances although a limited number of alleles were detected. Although the limited resolution of agarose gels likely causes some bias, profiling of larger sets with the individual plant approach appears feasible and more informative compared to the bulk analysis we conducted.
机译：大约65年前，收集并保存了150多个Swiss石类型的瑞士玉米地方品种（Zea mays L. ssp。mays）。由于阿尔卑斯山气候和文化的多样性，人们认为这种材料具有相当大的遗传多样性。为了证明这一点，需要一种有效的方法对瑞士基因库中的所有种质进行基因分析。选择简单的序列重复标记（SSR）分析，并在琼脂糖凝胶上观察聚合酶链反应（PCR）产物。这里分析了一组19种不同的地方品种，以：（i）研究其遗传多样性，（ii）研究并显示种群结构，以及（iii）确定是否可以使用DNA体积而不是单株植物进行此类分析。发现一个重复的四个重复样本彼此之间的距离比其他重复的样本更接近。此外，鉴定了几个登录的特定等位基因。大量DNA样品的PCR产物仅代表个体分析所揭示变异的一小部分。具有四个碱基重复基序的基因座在本体分析中比具有其他重复基序的基因座表现更好。分别基于个体和主体的分析，遗传距离矩阵之间的相关性很显着。因此，单一植物方法允许种质充分分化，尽管检测到的等位基因数量有限，但琼脂糖凝胶上可见的DNA体积导致相关的遗传距离。尽管琼脂糖凝胶的有限分辨率可能会引起一些偏差，但是与我们进行的批量分析相比，使用单个植物方法对较大的集合进行概要分析似乎是可行的，而且信息量更大。

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