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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting
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Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting

机译：基于ISSR指纹图谱的濒危菊科柑桔遗传变异。

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摘要

Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened, and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245 ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity. At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis (I broken vertical bar st = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large effective population sizes.
机译：洪河柑橘是中国云南东南部的洪河地区特有的极度濒危物种。使用简单序列间重复（ISSR）标记研究了其遗传多样性和分化。筛选了一百个引物，并通过13种信息可靠的引物从7个自然种群中扩增了总共245个基因座。在这245个ISSR位点中，有233个是多态性的，检测到的变异表明种内遗传多样性相对较高。在人群水平上，多态性位点（PPB）的平均百分比为36.50％，而平均预期杂合度（He）和香农多样性指数（Ho）分别为0.1327和0.1972。在物种水平上（在所有种群中），PPB为95.10％，而He和Ho分别为0.3520和0.5195。高Gst值（0.6247）表示人群之间存在显着差异，这一点已通过AMOVA分析得到了证实（I垂直竖线st = 0.6420）。群体之间的成对遗传同一性（I）值介于0.6341至0.7675之间，平均值为0.7008。我们认为，高水平的遗传分化可能是栖息地破碎化和有限的基因流的结果（Nm = 0.1502）。为了有效地进行原位保护和红C种群恢复，重要的是要保持历史进程，包括高配种率，充足的基因流和较大的有效种群规模。

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