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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic variation and relationships of pedigree-known oat, wheat, and barley cultivars relcavcd by bulking and single-plant sampling
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Genetic variation and relationships of pedigree-known oat, wheat, and barley cultivars relcavcd by bulking and single-plant sampling

机译：通过散装和单株采样法鉴定的家系已知燕麦，小麦和大麦品种的遗传变异及相关性

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Applications of bulking procedures have played an increasingly important role in molecular characterization of plant germplasm, but little attention has been made to address the effectiveness of detecting genetic variation and inferring genetic relationships via bulking. An analysis was performed here to compare the genetic variation detected and genetic relationships inferred via bulking and single-plant sampling of five oat (Avena saliva L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) cultivars with known pedigrees using amplified fragment length polymorphism (AFLP) markers. Three AFLP primer pairs were applied to screen one bulk and eight single-plant samples of each cultivar and up to 140 AFLP bands were scored for each sample. Analyses of these AFLP data showed bulking revealed AFLP variation up to 21.4% less than corresponding single-plant sampling for these crop species and also introduced up to 2.2% upward and 5.1% downward biases in detecting AFLP variations for each cultivar. The genetic relationships inferred by bulking using the Dice's coefficient, the simple matching coefficient, and the Jaccard's coefficient were largely the same, but differed from those in single-plant samples employing average Dice's coefficient,average simple matching coefficient, and AMOVA-based distance method. All of the inferred genetic relationships were not congruent to the known pedigrees. Clearly, substantial biases could exist in detection of AFLP variation and in inference of geneticrelationships from bulk samples, even for closely related germplasm, and more efforts to assess the effectiveness of bulking in inferring genetic variation and relationships are needed for more informative molecular characterization of plant germplasm.
机译：扩增程序的应用在植物种质的分子表征中起着越来越重要的作用，但是很少有人关注通过扩增来检测遗传变异和推断遗传关系的有效性。此处进行了分析，以比较通过对五个燕麦（Avena saliva L.），小麦（Triticum aestivum L.）和大麦（Hordeum vulgare L.）品种进行散装和单株取样检测到的遗传变异和遗传关系。使用扩增片段长度多态性（AFLP）标记的已知谱系。应用三对AFLP引物对筛选一个大块，每个品种八份单株样品，并为每个样品评分多达140条AFLP条带。对这些AFLP数据的分析表明，散装表明这些作物种类的AFLP变异比相应的单株采样少21.4％，并且在检测每个品种的AFLP变异时引入了高达2.2％的向上和5.1％的向下偏差。通过使用Dice系数，简单匹配系数和Jaccard系数进行扩增推断的遗传关系基本相同，但与使用平均Dice系数，平均简单匹配系数和基于AMOVA的距离方法的单株样品中的遗传关系不同。 。所有推断的遗传关系均与已知的血统书不符。显然，即使对于紧密相关的种质，在检测AFLP变异和从大样本中推断遗传关系时，也可能存在重大偏差，需要更多的努力来评估扩增在推断遗传变异和相关性方面的有效性，以便对植物进行更多的分子表征。种质。

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