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首页> 外文期刊>Genetic Resources and Crop Evolution >Polymorphism and genotype-specific markers for Dichanthium identified by random amplified polymorphic DNA.
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Polymorphism and genotype-specific markers for Dichanthium identified by random amplified polymorphic DNA.

机译：双歧杆菌的多态性和基因型特异性标记通过随机扩增的多态性DNA鉴定。

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摘要

One hundred decamer arbitrary primers were tested for PCR based amplification of seven genotypes (IG2208-S-1, IG2177, IG2180, IG2178, IG2165-S-1-1, IG2165-1 and Local-1) of an apomictic grass, Dichanthium annulatum, with the aim of screening polymorphic primers and genotype-specific markers. Out of 100 decamer primers tested, 42 produced no amplification or smeared non scorable bands, 12 amplified only single band and 46 yielded more than one polymorphic bands. Thirty-two primers out of 46 selected showed high level of polymorphism, producing 3-15 reproducible bands each for the seven Dichanthium genotypes examined. Among the total of 307 amplified fragments 222 were polymorphic, 53 bands were unique to the genotypes and 32 were monomorphic. Thus, with selected primers sufficient polymorphism could be detected to allow identification of individual genotypes. Genetic similarities of RAPD profiles generated were estimated via a coefficient of DICE and then the data were processed by cluster analysis (UPGMA). The maximum similarities between two genotypes (IG2180 and IG2178) was 58% and these two made a cluster with genotype IG2177 having similarity of only 54%. It clearly corroborated existence of high levels of polymorphism in this grass though being apomictic in nature. Primers like OPE-16, OPG-02, OPG-18, OPH-05, OPH-09, OPH-16, OPI-07 and OPF-06 found most informative as they produced specific bands pertaining to five out of seven genotypes. Polymerase chain reaction (PCR) offers a substantially simple, rapid and reliable method for identification of large number of Dichanthium genotypes once enough number of reproducible and suitable primers is screened..
机译：测试了100个十聚体任意引物，以基于PCR的方法对无融合生殖草，圆环双歧杆菌的7个基因型（IG2208-S-1，IG2177，IG2180，IG2178，IG2165-S-1-1，IG2165-1和Local-1）进行扩增。 ，目的是筛选多态性引物和基因型特异性标记。在所测试的100个decamer引物中，有42条未产生扩增或涂上不可评分的条带，仅12条扩增了单条带，有46条产生了一个以上的多态性条带。从46个所选引物中，有32个引物显示出高水平的多态性，对于所检查的7个Dichanthium基因型，每个引物均产生3-15条可再现的条带。在总共307个扩增片段中，有222个是多态性的，有53个条带是基因型特有的，有32个是单态性的。因此，使用选择的引物，可以检测到足够的多态性以允许鉴定单个基因型。通过DICE系数估算产生的RAPD图谱的遗传相似性，然后通过聚类分析（UPGMA）处理数据。两种基因型（IG2180和IG2178）之间的最大相似度为58％，这两个基因型与IG2177相似度仅为54％构成了一个集群。尽管本质上是无融合生殖的，但它显然证实了这种草中高水平多态性的存在。像OPE-16，OPG-02，OPG-18，OPH-05，OPH-09，OPH-16，OPI-07和OPF-06这样的引物发现的信息最多，因为它们产生了与7个基因型中的5个有关的特定条带。一旦筛选出足够数量的可复制和合适的引物，聚合酶链反应（PCR）将提供一种简单，快速和可靠的方法来鉴定大量双歧杆菌的基因型。

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