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Genetic factors determining varietal differences in characters affecting yield between two rice (Oryza sativa L.) varieties, Koshihikari and IR64

机译:决定两个水稻品种越光和IR64产量性状差异的遗传因素

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摘要

The yield of Koshihikari, a Japanese premium rice variety, is relatively lower than that of modern high yielding varieties. IR64 carries several well-known genes such as GS3, an important gene for grain size, sd-1, a semi-dwarf gene, and NARROW LEAF1 (NAL1), a gene for small, narrow flag leaves. In this study, we used two sets of chromosome segment substitution lines (CSSLs), from Koshihikari and IR64, and attempted to evaluate the genetic factors that cause differences between parents by analyzing the function of chromosome regions affecting a trait (CRATs). For 28 traits, we identified 312 CRATs in the Koshihikari background and 275 in the IR64 background. In these, donor alleles had positive effects in 84 and 103 CRATs, respectively. Among these, the CRAT related to GS3 and those for grain number expanded the potential sink size in Koshihikari, although this did not affect final yield. The combination of CRATs that enhances source ability may increase grain yield. Although the sd-1 gene might improve resistance to lodging, the yield of CSSLs with sd-1 decreased by 28.7 %. These results suggest that the smaller biomass conferred by sd-1 might reduce canopy photosynthesis. In the Koshihikari background, the CRAT related to NAL1 and those located on chr. 6 increased SPAD value but had the opposite effect on leaf size. Two CRATs that were detected on chr. 6 and 7 increased leaf area without any effect on the SPAD value. The combination of these CRATs for area and SPAD value might improve source ability.
机译:日本优质水稻品种越光的产量相对低于现代高产品种。 IR64带有几个著名的基因,例如GS3(一个重要的晶粒尺寸基因),sd-1(一个半矮基因)和NARROW LEAF1(NAL1)(一个狭窄的小旗叶基因)。在这项研究中,我们使用了来自越光(Koshihikari)和IR64的两组染色体片段替换系(CSSLs),并试图通过分析影响性状的染色体区域(CRAT)的功能来评估造成父母之间差异的遗传因素。对于28个性状,我们在Koshihikari背景中鉴定了312个CRAT,在IR64背景中鉴定了275个CRAT。在这些中,供体等位基因分别在84和103个CRAT中具有积极作用。其中,与GS3有关的CRAT和与粒数有关的CRAT扩大了越光的潜在库规模,尽管这并不影响最终产量。增强来源能力的CRAT组合可以提高谷物产量。尽管sd-1基因可能会提高抗倒伏能力,但带有sd-1的CSSL的产量下降了28.7%。这些结果表明,sd-1赋予的较小的生物量可能会减少冠层的光合作用。在越光(Koshihikari)背景下,CRAT与NAL1和位于chr的那些相关。 6增加SPAD值,但对叶大小有相反的影响。在chr上检测到两个CRAT。 6和7增加了叶面积,而对SPAD值没有任何影响。这些CRAT的面积和SPAD值的组合可能会提高源能力。

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