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首页> 外文期刊>Genetic Resources and Crop Evolution >Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.
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Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.

机译：内部转录的核糖体DNA间隔区序列解决了蚕豆属的复杂分类历史。

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摘要

The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study.
机译：对18S-28S nrDNA的ITS1-5.8S-ITS2区进行了测序，从两个亚属的9个部分中选择了49个蚕豆属和亚种，以解决分类学上的矛盾。来自内部转录间隔区（ITS）序列的系统发生学强烈支持以下观点：蚕豆和蚕豆都远离蚕豆节中的Narbonensis物种复合体（NSC）。蚕豆与Peregrinae和Hypechusa区域的排列更紧密，而bithynica蚕与Vicia区域的排列更紧密。 NSC特有的六个同形同构替换支持Narbonensis复合体的单独部分。野豌豆（Vicia johannis）子亚种。约翰尼斯（Johannis）是新南威尔士州（NSC）物种最多的物种，其之间的遗传距离很高。约翰尼斯以及NSC的其余物种和亚种。这些结果表明，Vicia johannis亚种。约翰尼斯应该被赋予物种等级。邻近的和基于简约的树木支持部分野豌豆（在排除野豌豆后）和克拉科部分（在野豌豆和野豌豆后）。根据目前的ITS序列分类分析，Hypechusa部分属于多系和副系。本研究支持该属的祖先数x = 7。

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