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首页> 外文期刊>Genetic Resources and Crop Evolution >Comparative analysis of organelle DNAs in acid citrus grown in Japan using PCR-RFLP method
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Comparative analysis of organelle DNAs in acid citrus grown in Japan using PCR-RFLP method

机译：PCR-RFLP法对日本酸性柑橘中细胞器DNA的比较分析

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PCR-RFLP analyses of three regions for each of chloroplast DNA (cpDNA; rbcL-ORF106, trnD-trnT, trnH-trnK) and mitochondrial DNA (mtDNA; nad7/exon2-exon3, nad7/exon3-exon4, 18S-5S) were performed in 26 cultivars of acid citrus grown in Japan to identify polymorphisms and classify them. The polymorphisms were compared with those of three true Citrus species, i.e., mandarin, pummelo and citron. Ichang papeda (C. ichangensis) was also included in this study to find its relationship with Yuzu. Inter-species cpDNA variation was recognized and the acid citrus were divided into three groups, namely; I ('Yuzukichi' and 'Kinkoyu'), II [sour oranges ('Kaiseito', 'Daidai' and 'China daidai'), 'Nansho daidai', 'Kiku daidai', C. sudachi ('Mushi yukaku', 'Yushi yukaku' and 'Yushi mukaku'), C. sphaerocarpa ('Kabosu' and 'Aka kabosu'), C. kizu ('Taninaka kizu', 'Kinosu' and 'Kizu'), 'Zanbo', 'Mochiyu', 'Jabara' and 'Naoshichi'], and III [Yuzu ('Tetraploid', 'Tochikei yuzu' and 'Yamanekei yuzu'), 'Matsuda sudachi', 'Zuishoyu', 'Hanayu' and 'Yuko']. CpDNA restriction patterns of the three true Citrus species differed from each other as well as from those of ichang papeda. CpDNA restriction patterns of group I of the acid citrus were identical to those of mandarins. Group II showed the same as pummelos. CpDNA restriction patterns of group III were differed from those of the three true Citrus species in the three regions. This group was differed from ichang papeda after digestion of trnH-trnK PCR products with TaqI, HinfI and AluI, while they showed identical restriction patterns in two regions, rbcL-ORF106 and trnD-trnT. Citrons and ichang papeda were placed in groups IV and V, respectively. Based on mtDNA restriction patterns, the acid citrus were divided into three groups; i, ii and iii. In groups i and ii accessions of groups I and II of cpDNA were placed with mandarins and pummelos, respectively. In group iii accessions of group III of cpDNA were placed with ichang papeda. Citrons were placed in a distinct group, iv.
机译：对每个叶绿体DNA（cpDNA; rbcL-ORF106，trnD-trnT，trnH-trnK）和线粒体DNA（mtDNA; nad7 / exon2-exon3，nad7 / exon3-exon4，18S-5S）的三个区域进行PCR-RFLP分析。该研究在日本生长的26个酸性柑橘品种中进行，以鉴定多态性并将其分类。将其多态性与三种真实柑橘属物种，即普通话，柚子和柚子进行了比较。这项研究还包括伊昌木瓜（C. ichangensis），以发现其与柚子的关系。认识到种间cpDNA的变化，并将酸性柑桔分为三组： I（“ Yuzukichi”和“ Kinkoyu”），II（酸橙（“ Kaiseito”，“ Daidai”和“ China daidai”），“ Nansho daidai”，“ Kiku daidai”，C。sudachi（“ Mushi yukaku”，“ Yushi yukaku'和'Yushi mukaku'），s.sphaerocarpa（'Kabosu'和'Aka kabosu'），C。kizu（'Taninaka kizu'，'Kinosu'和'Kizu'），'Zanbo'，'Mochiyu'， “ Jabara”和“ Naoshichi”）和III [Yuzu（“四倍体”，“ Tochikei yuzu”和“ Yamanekei yuzu”），“ Matsuda sudachi”，“ Zuishoyu”，“ Hanayu”和“ Yuko”。这三个真正的柑橘物种的CpDNA限制模式彼此不同，也不同于伊昌木瓜。酸性柑橘I组的CpDNA限制模式与普通话相同。第二组显示与柚子相同。第三组的CpDNA限制模式与三个地区的三个真实柑橘属物种不同。在用TaqI，HinfI和AluI消化trnH-trnK PCR产物后，该组与ichang papeda不同，尽管它们在rbcL-ORF106和trnD-trnT两个区域显示相同的限制性酶切模式。柚子和宜昌辣椒粉分别置于IV和V组。根据线粒体DNA限制模式，将酸性柑桔分为三组： i，ii和iii。在第i和第ii组中，分别将cpDNA的第I和II组的登录物与普通话和柚一起放置。在第III组中，将cpDNA的第III组的种质与ichang papeda一起放置。将香tron放置在不同的组中。

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