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首页> 外文期刊>Genetic Resources and Crop Evolution >Results of molecular analysis of an archaeological hemp (Cannabis sativa L.) DNA sample from North West China
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Results of molecular analysis of an archaeological hemp (Cannabis sativa L.) DNA sample from North West China

机译：中国西北地区考古大麻（Cannabis sativa L.）DNA样本的分子分析结果

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摘要

Hemp (Cannabis sativa L.) cultivation and utilization is an ancient practice to human civilization. There are some controversies on the origin and subsequent spread of this species. Ancient plant DNA has proven to be a powerful tool to solve phylogenetic problems. In this study, ancient DNA was extracted from an archaeological specimen of Cannabis sativa associated with archaeological human remains from China. Ribosomal and Cannabis specific chloroplast DNA regions were PCR amplified. Sequencing of a species-specific region and subsequent comparison with published sequences were performed. Successful amplification, sequencing and sequence comparison with published data suggested the presence of hemp specific DNA in the archeological specimen. The role of Humulus japonicus Sieb. et Zucc. in the evolution of Cannabis is also indicated. The identification of ancient DNA of 2500 years old C. sativa sample showed that C. sativa races might have been introduced into China from the European-Siberian center of diversity.
机译：大麻（Cannabis sativa L.）的种植和利用是人类文明的古老习俗。关于该物种的起源和随后的传播存在一些争议。事实证明，古代植物DNA是解决系统发育问题的有力工具。在这项研究中，从与中国考古遗址相关联的大麻的考古标本中提取了古代DNA。核糖体和大麻的特定叶绿体DNA区域进行了PCR扩增。进行物种特异性区域的测序，然后与已发表的序列进行比较。成功的扩增，测序和与已发表数据的序列比较表明，考古标本中存在大麻特异性DNA。 Hum草的作用。 et Zucc。在大麻的进化中也有说明。 2500年古老的苜蓿样品的古代DNA鉴定表明，苜蓿种可能已从欧洲-西伯利亚的多样性中心引入中国。

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