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首页> 外文期刊>Genetic Resources and Crop Evolution >The Frankincense tree (Boswellia sacra, Burseraceae) from Oman: ITS and ISSR analyses of genetic diversity and implications for conservation
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The Frankincense tree (Boswellia sacra, Burseraceae) from Oman: ITS and ISSR analyses of genetic diversity and implications for conservation

机译：来自阿曼的乳香树（乳香，蔷薇科）：遗传多样性的ITS和ISSR分析及其对保护的意义

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摘要

DNA sequences from the ITS region of the nuclear genome and Inter-Simple Sequence Repeat markers (ISSR) were used to estimate genetic diversity among and within populations of the Frankincense tree Boswellia sacra from Dhofar, Oman. This is a culturally and ecologically relevant species that is showing symptoms of decline due to anthropic factors and, possibly, global warming. ITS sequences were 511 bp long and showed low (6.4%) variation among geographically different populations. The four selected ISSR primers yielded 93 reproducible bands, of which 91 (97.9%) were polymorphic in the 97 individual profiles obtained. Total genetic diversity (H T) and average heterozygosity within populations (H S) resulted fairly low (0.22 and 0.136, respectively). The accession from Wadi Dowkah, an UNESCO world heritage site, showed the lowest level of genetic diversity (H E = 0.107), while the eastern populations from the Hasik area harboured a slightly greater amount of variation. Analysis of Molecular Variance showed that differentiation among populations was relatively high (38.1%), possibly due to the reduced gene flow between the largely isolated stands of Boswellia (N m = 0.39). Genetic distances and AMOVA suggested a clear differentiation between the eastern and western coastal populations, while those from the internal area did not form a consistent group. For conservation, the eastern sites should be given priority as core populations harbouring significant amounts of allelic diversity. Reasons for reinforcing the more depauperated stands, such as Wadi Dowkah, with local plant material only or, alternatively, with the introduction of germplasm from genetically distinct stands are discussed.
机译：来自核基因组ITS区域的DNA序列和简单序列间重复标记（ISSR）被用于估算阿曼Dhofar乳香树Boswellia sacra种群之间和种群内部的遗传多样性。这是一种与文化和生态相关的物种，由于人为因素以及可能的全球气候变暖而表现出下降的症状。 ITS序列长511 bp，在不同地理区域的人群中显示较低的变异（6.4％）。所选择的四种ISSR引物产生93条可重复条带，其中91条（97.9％）在获得的97个独立谱中具有多态性。总的遗传多样性（H T）和种群内的平均杂合度（H S）相当低（分别为0.22和0.136）。联合国教科文组织世界遗产瓦迪·道卡（Wadi Dowkah）的遗存显示出最低的遗传多样性水平（H E = 0.107），而哈西克地区的东部人口则存在稍多的变异。分子方差分析表明，种群之间的分化程度相对较高（38.1％），这可能是由于在很大程度上隔离的乳香林分之间的基因流减少（N m = 0.39）。遗传距离和AMOVA表明东部和西部沿海种群之间有明显的区别，而内部种群则没有形成一致的群体。为了保护，应优先考虑东部地区，因为它们是拥有大量等位基因多样性的核心种群。讨论了仅使用本地植物材料或通过遗传上不同的林分引入种质来加强更枯萎的林分的原因，例如Wadi Dowkah。

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