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首页> 外文期刊>Genetic Resources and Crop Evolution >Traditional seed management and genetic diversity in barley varieties in high-hill agro-ecosystems of Nepal.
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Traditional seed management and genetic diversity in barley varieties in high-hill agro-ecosystems of Nepal.

机译：尼泊尔高丘陵农业生态系统中大麦品种的传统种子管理和遗传多样性。

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摘要

Chawali and Lekali are two common farmer's barley varieties or landraces in Jumla, Nepal (2,240-3,000 m) with small to bold grains and wide adaptation from irrigated low lands to high hills. This study was undertaken to test whether features of the traditional seed system can significantly influence the diversity of a crop and its conservation on-farm. In Jumla (high-hill), the barley seed system is completely informal and is mainly from farmer to farmer. In the present study, the seed flows and the pattern of genetic diversity in barley were investigated to detect differences between the two varieties and test the divergence among populations of each variety These data suggested that Chawali, the more common variety, was less subject to homogenising gene flow between farms than was Lekali. A total of 128 farming households were surveyed for seed supply information and 128 populations for each landrace from two villages: Kartikswami and Talium were collected for SSR diversity analysis. Some 92 SSRs were screened in an initial sample of 20 barley populations of both landraces and 2 improved varieties (LG-51 and Soluwa). Of the 81 SSRs that consistently amplified, only 15 SSRs (19%) were polymorphic with gene diversity values ranging from 0.09 to 0.71. A medium to low diversity was detected among the landrace populations of barley varieties. Chawali populations were less polymorphic within ecological groups, and more divergent between than were Lekail populations. This result accords with Chawali having a more conservative local seed system.Digital Object Identifier http://dx.doi.org/10.1007/s10722-011-9689-2
机译：Chawali和Lekali是尼泊尔Jumla（2,240-3,000 m）的两种常见的农民大麦品种或地方品种，籽粒小而粗，适应范围从灌溉低地到高丘陵。进行这项研究是为了检验传统种子系统的特征是否可以显着影响农作物的多样性及其在农场的保护。在Jumla（高坡），大麦种子系统是完全非正式的，主要是从农民到农民。在本研究中，对大麦的种子流和遗传多样性模式进行了研究，以检测两个品种之间的差异并测试每个品种的种群之间的差异。这些数据表明，Chawali（较常见的品种）受均质化的影响较小。农场之间的基因流比莱卡利还多。总共对128个农户进行了种子供应信息调查，并从两个村庄Kartikswami和Talium收集了每个地方种的128个种群用于SSR多样性分析。在最初的20个大麦品种和2个改良品种（LG-51和Soluwa）的大麦种群样本中筛选了约92个SSR。在连续扩增的81个SSR中，只有15个SSR（19％）是多态的，基因多样性值在0.09到0.71之间。在大麦品种的地方品种种群中检测到中等至低多样性。与Lekail种群相比，Chawali种群在生态群内的多态性较少，并且在不同种群之间的差异更大。此结果与Chawali具有更保守的本地种子系统相符。数字对象标识符http://dx.doi.org/10.1007/s10722-011-9689-2

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