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首页> 外文期刊>Genome >A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation
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A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation

机译：基于AFLP标记的侧耳侧耳的遗传连锁图谱以及无孢子突变基因区域的定位

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摘要

In the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel., the enormous number of spores produced by fruiting bodies can adversely affect mushroom growers' health, mushroom cultivation facilities, and the genetic diversity of natural populations. In this study, we constructed a primary genetic linkage map and identified the locus associated with the sporulation-deficient (sporeless) mutation of P. pulmonarius using 150 progeny isolates derived from a cross between sporeless and wild-type isolates. Based on the segregation of 300 AFLP markers, two mating-type factors, and the sporeless trait, a linkage map was generated consisting of 12 linkage groups. The map covered a total ge-netic distance of 971 cM, with an average marker interval of 5.2 cM. The gene region responsible for the sporeless muta-tion was located in linkage group II including 40 AFLP markers and the A mating-type factor locus. Of these markers, the nearest marker to the sporeless locus was located 1.4 cM away. Construction of this P. pulmonarius genetic linkage map and identification of markers that are closely linked to the sporeless locus will facilitate marker-assisted selective breeding of a sporeless strain with economically important traits.
机译：在食用蘑菇中，包括平菇（Prurotus pulmonarius（Fr.）Quel。），子实体产生的大量孢子会对蘑菇种植者的健康，蘑菇种植设施和自然种群的遗传多样性产生不利影响。在这项研究中，我们构建了一个主要的遗传连锁图谱，并使用源自无孢子和野生型分离物之间杂交的150个后代分离物，鉴定了与肺炎假单胞菌的孢子形成缺陷（无芽孢）突变相关的基因座。基于300个AFLP标记，两个交配型因子和无孢子性状的分离，生成了一个由12个连锁组组成的连锁图。该图谱的总遗传距离为971 cM，平均标记间隔为5.2 cM。负责无孢子突变的基因区域位于包含40个AFLP标记和A交配型因子基因座的II型连锁群中。在这些标记中，最接近无孢子基因座的标记位于1.4 cM处。构建该肺炎假单胞菌遗传连锁图谱并鉴定与无孢子基因座密切相关的标记物，将有助于具有经济重要性状的无孢子菌株的标记物辅助选育。

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