...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetical Research >Phylogenetic relationships and genetic diversity of the USDA Vigna germplasm collection revealed by gene-derived markers and sequencing
【24h】

Phylogenetic relationships and genetic diversity of the USDA Vigna germplasm collection revealed by gene-derived markers and sequencing

机译：通过基因衍生标记和测序揭示USDA Vigna种质集合的系统发生关系和遗传多样性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phylogenetic relationships in the USDA Vigna germplasm collection are somewhat unclear and their genetic diversity has not been measured empirically. To reveal interspecific phylogenetic relationships and assess their genetic diversity, 48 accessions representing 12 Vigna species were selected, and 30 gene-derived markers from legumes were employed. Some high-quality amplicons were sequenced. Indels (insertion/deletions) were discovered from the sequence alignments that were specific identifiers for some Vigna species. With regard to revealing polymorphisms, intron-spanning markers were more effective than exon-derived markers. These gene-derived markers were more successful in revealing interspecific polymorphisms than intraspecific polymorphisms at both the DNA fragment and sequence levels. Two different dendrograms were generated from DNA fragment data and sequence data, respectively. The results from these two dendrograms Supported each other and showed similar phylogenetic relationships among the Vigna species investigated. The accessions clustered into four main groups and 13 subgroups. Each subgroup represents a subgenus or a species. Phylogenetic analysis revealed that an accession might be misclassified in our collection. The putative misclassified accession was further supported by seed morphology. Limited intraspecific genetic diversity was revealed by these gene-derived markers and/or sequences. The USDA Vigna germplasm collection currently consists of multiple species with many accessions further classified into specific subspecies, but very few Subspecies of the total subspecies available exist within the collection. Based on our results, more attention Should be paid to the subspecies, wild forms and/or botanical varieties for future curation in order to expand the genetic diversity of Vigna germplasm in the USDA collection.
机译：USDA Vigna种质收集中的亲缘关系尚不清楚，其遗传多样性尚未经过经验测量。为了揭示种间的系统发育关系并评估其遗传多样性，选择了代表12种Vigna物种的48个种质，并从豆类中提取了30个基因衍生的标记。测序了一些高质量的扩增子。从序列比对中发现插入缺失（插入/缺失），这些序列比对是某些Vi豆属的特异性标识符。关于揭示多态性，跨内含子的标记比外显子衍生的标记更有效。在DNA片段和序列水平上，这些基因衍生标记比种内多态性更能揭示种间多态性。分别从DNA片段数据和序列数据生成了两个不同的树状图。这两个树状图的结果相互支​​持，并显示了所研究的Vi豆属之间相似的系统发育关系。这些种质分为四个主要组和13个亚组。每个亚组代表一个亚属或一个物种。系统发育分析表明，在我们的馆藏中某种可能被错误分类。种子形态进一步支持推定的错误分类登录。这些基因衍生的标记和/或序列揭示出有限的种内遗传多样性。 USDA Vigna种质资源库目前由多个物种组成，许多种进一步分类为特定的亚种，但是集合中可利用的总亚种中只有很少的亚种。根据我们的研究结果，应该更多地注意亚种，野生形式和/或植物变种，以备将来管理，以扩大USDA集合中gna豆种质的遗传多样性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号