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DNA barcode variability and host plant usage of fruit flies (Diptera: Tephritidae) in Thailand

机译：泰国果蝇的DNA条码变异性和寄主植物的使用（双翅目：蝇科）

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摘要

The objectives of this study were to examine the genetic variation in fruit flies (Diptera: Tephritidae) in Thailand and to test the efficiency of the mitochondrial cytochrome c oxidase subunit I (COI) barcoding region for species-level identification. Twelve fruit fly species were collected from 24 host plant species of 13 families. The number of host plant species for each fruit fly species ranged between 1 and 11, with Bactrocera correcta found in the most diverse host plants. A total of 123 COI sequences were obtained from these fruit fly species. Sequences from the NCBI database were also included, for a total of 17 species analyzed. DNA barcoding identification analysis based on the best close match method revealed a good performance, with 94.4% of specimens correctly identified. However, many specimens (3.6%) had ambiguous identification, mostly due to intra- and interspecific overlap between members of the B. dorsalis complex. A phylogenetic tree based on the mitochondrial barcode sequences indicated that all species, except for the members of the B. dorsalis complex, were monophyletic with strong support. Our work supports recent calls for synonymization of these species. Divergent lineages were observed within B. correcta and B. tuberculata, and this suggested that these species need further taxonomic reexamination.
机译：这项研究的目的是检查泰国果蝇（双翅目：蝇科）的遗传变异，并测试线粒体细胞色素c氧化酶亚基I（COI）条形码区域对物种水平鉴定的效率。从13个科的24种寄主植物中收集了12种果蝇。每个果蝇物种的寄主植物种类数量在1至11之间，其中Bactrocera Correcta存在于最多样化的寄主植物中。从这些果蝇物种中总共获得了123个COI序列。还包括来自NCBI数据库的序列，共分析了17种。基于最佳紧密匹配法的DNA条形码识别分析显示出良好的性能，正确识别94.4％的标本。然而，许多标本（3.6％）具有不明确的识别，主要是由于背侧双歧杆菌复合体之间的种内和种间重叠。基于线粒体条形码序列的系统树表明，除了背果双歧杆菌复合体的成员外，所有物种都是单系的，并具有强大的支持力。我们的工作支持最近呼吁将这些物种同义化。在B.correcta和B.tuberculata中观察到不同的谱系，这表明这些物种需要进一步的分类学重新审查。

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