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首页> 外文期刊>Genome >QTL mapping of early flowering and resistance to ascochyta blight in chickpea
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QTL mapping of early flowering and resistance to ascochyta blight in chickpea

机译：鹰嘴豆早开花和抗灰霉病的QTL定位

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In western Canada, chickpea (Cicer arietinum L.) production is challenged by short growing seasons and infestations with ascochyta blight. Research was conducted to determine the genetic basis of the association between flowering time and reaction to ascochyta blight in chickpea. Ninety-two chickpea recombinant inbred lines (RILs) developed from a cross between ICCV 96029 and CDC Frontier were evaluated for flowering responses and ascochyta blight reactions in growth chambers and fields at multiple locations and during several years. A wide range of variation was exhibited by the RILs for days to flower, days to maturity, node of first flowering, plant height, and ascochyta blight resistance. Moderate to high broad sense heritability was estimated for ascochyta blight reaction (H-2 = 0.14-0.34) and for days to flowering (H-2 = 0.45-0.87) depending on the environments. Negative correlations were observed among the RILs for days to flowering and ascochyta blight resistance, ranging from r = -0.21 (P < 0.05) to -0.58 (P < 0.0001). A genetic linkage map consisting of eight linkage groups was developed using 349 SNP markers. Seven QTLs for days to flowering were identified that individually explained 9%-44% of the phenotypic variation. Eight QTLs were identified for ascochyta blight resistance that explained phenotypic variation ranging from 10% to 19%. Clusters of QTLs for days to flowering and ascochyta blight resistances were found on chromosome 3 at the interval of 8.6-23.11 cM and on chromosome 8 at the interval of 53.88-62.33 cM.
机译：在加拿大西部，鹰嘴豆（Cicer arietinum L.）的生产受到生长季节短和稻草枯萎病侵袭的挑战。进行了研究以确定鹰嘴豆开花时间与对枯萎病的反应之间的关联的遗传基础。评估了从ICCV 96029和CDC Frontier杂交产生的92个鹰嘴豆重组自交系（RIL），用于在多个地点和数年的生长室和田地中的开花反应和稻草枯萎病反应。 RILs在开花天数，成熟天数，初开花节，植物高度和抗灰霉病等方面表现出广泛的变化。视环境而定，对灰暗病反应（H-2 = 0.14-0.34）和开花期（H-2 = 0.45-0.87）估计了中等至高度的遗传力。 RIL之间持续数天与开花和抗灰霉病抗性之间呈负相关，范围从r = -0.21（P <0.05）到-0.58（P <0.0001）。使用349个SNP标记开发了由八个连锁组组成的遗传连锁图谱。鉴定出开花前几天的七个QTL，分别解释了9％-44％的表型变异。确定了八个QTL的抗灰霉病抗性，这解释了表型变异范围从10％到19％。在3号染色体上以8.6-23.11 cM的间隔和8号染色体上以53.88-62.33 cM的间隔发现了对开花和抗灰霉病抗性数天的QTL簇。

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