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首页> 外文期刊>Bulletin of the European Association of Fish Pathologists >First report of Flavobacterium psychrophilum isolated from cultured rainbow trout (Oncorhynchus mykiss) in Argentina
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First report of Flavobacterium psychrophilum isolated from cultured rainbow trout (Oncorhynchus mykiss) in Argentina

机译：从阿根廷养殖的虹​​鳟鱼（Oncorhynchus mykiss）中分离出嗜黄杆菌的首次报道

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摘要

Sub-acute mortality of farmed rainbow trout, Oncorhynchus mykiss, associated with symptoms of dermal necrosis has been often noticed by fish farmers in reservoirs of North Patagonia, Argentina. However, the causative agent of this problem has not been identified. In this work, rainbow trout, which were moribund and showing dermal necrosis on the body surface, were collected from a hatchery and fish farms in Neuquen province. Yellow pigment producing bacteria on KDM-2 agar plates were isolated from the kidneys of those fish. These bacteria grew on KDM-2 agar plates but not in other culture media at the primary isolation. The results from PCR analysis and biochemical characterization indicate that these isolates can be determined as Flavobacterium psychrophilum. Results from two challenge tests, performed by rearing fish under overcrowded conditions and by intraperitoneal injection of bacteria, suggest virulence of the isolated bacteria to juvenile rainbow trout and their ability to cause disease. This is the first report of F. psychrophilum in Argentina.
机译：在阿根廷北巴塔哥尼亚的水库中，养鱼户经常注意到养殖虹鳟鱼的亚急性死亡率，并伴有皮肤坏死症状。但是，尚未确定此问题的病因。在这项工作中，虹鳟鱼死于内乌肯省的一家孵化场和养鱼场，这些虹鳟鱼濒临灭绝并在体表显示出皮肤坏死。从这些鱼的肾脏中分离出在KDM-2琼脂平板上产生黄色素的细菌。这些细菌在最初分离时在KDM-2琼脂平板上生长，但不在其他培养基中生长。 PCR分析和生化特征的结果表明，这些分离物可以被确定为嗜冷黄杆菌。通过在拥挤的条件下饲养鱼并通过腹膜内注射细菌进行的两个挑战性试验的结果表明，分离出的细菌对幼年虹鳟的毒性及其引起疾病的能力。这是阿根廷F. psychrophilum的首次报道。

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