...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genome >Characterization of microRNAs and their targets in wild barley (Hordeum vulgare subsp spontaneum) using deep sequencing
【24h】

Characterization of microRNAs and their targets in wild barley (Hordeum vulgare subsp spontaneum) using deep sequencing

机译：使用深度测序表征野生大麦（Hordeum vulgare subsp spontaneum）中的microRNA及其靶标

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNAs (miRNA) are a class of small, endogenous RNAs that play a negative regulatory role in various developmental and metabolic processes of plants. Wild barley (Hordeum vulgare subsp. spontaneum), as the progenitor of cultivated barley (Hordeum vulgare subsp. vulgare), has served as a valuable germplasm resource for barley genetic improvement. To survey miRNAs in wild barley, we sequenced the small RNA library prepared from wild barley using the Illumina deep sequencing technology. A total of 70 known miRNAs and 18 putative novel miRNAs were identified. Sequence analysis revealed that all of the miRNAs identified in wild barley contained the highly conserved hairpin sequences found in barley cultivars. MiRNA target predictions showed that 12 out of 52 miRNA families were predicted to target transcription factors, including 8 highly conserved miRNA families in plants and 4 wheat-barley conserved miRNAfamilies. In addition to transcription factors, other predicted target genes were involved in diverse physiological and metabolic processes and stress defense. Our study for the first time reported the large-scale investigation of small RNAs in wild barley, which will provide essential information for understanding the regulatory role of miRNAs in wild barley and also shed light on future practical utilization of miRNAs for barley improvement.
机译：微小RNA（miRNA）是一类小的内源性RNA，它们在植物的各种发育和代谢过程中起负调控作用。作为栽培大麦（Hordeum vulgare subsp。vulgare）的祖先，野生大麦（Hordeum vulgare subsp。vulgare）已成为大麦遗传改良的宝贵种质资源。为了调查野生大麦中的miRNA，我们使用Illumina深度测序技术对从野生大麦制备的小RNA文库进行了测序。总共鉴定出70种已知的miRNA和18种推定的新型miRNA。序列分析显示，在野生大麦中鉴定出的所有miRNA均包含在大麦品种中发现的高度保守的发夹序列。 MiRNA靶标预测表明，预测52个miRNA家族中有12个靶向转录因子，包括植物中8个高度保守的miRNA家族和4个小麦-大麦保守的miRNA家族。除转录因子外，其他预测的靶基因也参与了各种生理和代谢过程以及应激防御。我们的研究首次报道了对野生大麦中小RNA的大规模研究，这将为了解miRNA在野生大麦中的调控作用提供必要的信息，并为miRNA在大麦改良中的实际应用提供了启示。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号