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首页> 外文期刊>Genome >Comparative transcriptome analysis of yeast strains carrying slt2, rlm1, and pop2 deletions
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Comparative transcriptome analysis of yeast strains carrying slt2, rlm1, and pop2 deletions

机译：带有slt2，rlm1和pop2缺失的酵母菌株的比较转录组分析

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摘要

The function of the genes SLT2 (encoding the Mpk1 protein), RLM1, and POP2 have previously been related to several stress responses in yeasts. DNA arrays have been used to identify differences among the transcriptomes of a Saccharomyces cerevisiae wild type strain and its derivative Delta slt2, Delta rlm1, and Delta pop2 mutants. Correspondence analyses indicate that the vast majority of genes that show lower expression in Delta rlm1 also show lower expression in Delta slt2. In contrast, there is little overlap between the results of the transcriptome analyses of the Delta pop2 strain and the Delta slt2 or Drlm1 strains. The DNA array data were validated by reverse Northern blotting and chromatin immunoprecipitation (ChIp). ChIp assays demonstrate Rlm1p binding to specific regions of the promoters of two genes that show expression differences between the Delta rlm1 mutant and wild type strains. Interestingly, RLM1 deletion decreases the transcription of SLT2, encoding the Mpk1p kinase that phosphorylates Rlm1p, suggesting a feedback control in the signal transduction pathway. Also, deletion of RLM1 causes a decrease in the mRNA level of KDX1, which is paralogous to SLT2. In contrast, deletion of POP2 is accompanied by an increase of both SLT2 and KDX1 levels. We show that SLT2 mRNA increase in the Dpop2 strain is due to a decrease in RNA turnover, consistent with the expected loss of RNA-deadenylase activity in this strain.
机译：SLT2基因（编码Mpk1蛋白），RLM1和POP2的功能以前与酵母中的几种应激反应有关。 DNA阵列已用于鉴定酿酒酵母野生型菌株及其衍生的Delta slt2，Delta rlm1和Delta pop2突变体的转录组之间的差异。对应分析表明，绝大多数在Delta rlm1中显示较低表达的基因在Delta slt2中也显示较低表达。相比之下，Delta pop2菌株和Delta slt2或Drlm1菌株的转录组分析结果之间几乎没有重叠。通过反向Northern印迹和染色质免疫沉淀（ChIp）验证了DNA阵列数据。 ChIp分析表明Rlm1p与两个基因启动子的特定区域结合，这两个基因在Delta rlm1突变体和野生型菌株之间表现出表达差异。有趣的是，RLM1缺失减少了SLT2的转录，该转录编码磷酸化Rlm1p的Mpk1p激酶，表明信号转导途径中存在反馈控制。此外，RLM1的缺失会导致KDX1的mRNA水平下降，这与SLT2相似。相反，删除POP2伴随SLT2和KDX1水平的增加。我们表明，Dpop2菌株中SLT2 mRNA的增加是由于RNA周转率的下降，与该菌株中RNA-腺苷酸酶活性的预期损失相一致。

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