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首页> 外文期刊>Genes to cells : >Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament.
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Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament.

机译：Fhos2是一种新型的与FORMIN相关的肌动蛋白组织蛋白，可能与Nestin中间丝有关。

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摘要

Fhos1 is a mammalian formin-family protein, and functions as an organizer of the actin microfilament. Here we have cloned human and mouse cDNAs for a novel Fhos homolog, designated Fhos2. The messages for Fhos2 are expressed in the heart, kidney, and brain, where the Fhos1 mRNAs are not abundant. Two splice variants of Fhos2 exist in a tissue-specific manner; the longer variant Fhos2L is the major form in the heart, whereas the kidney and brain predominantly express Fhos2S that encodes a shorter protein. Over-expression of an active form of the two Fhos2 variants, as well as that of Fhos1, induces the formation of actin stress fibers in HeLa cells, suggesting that Fhos2 acts as an actin-organizing protein. Biochemical analysis using rat cardiomyoblastic H9c2 (2-1) cells reveals that endogenous Fhos2 is enriched in the intermediate filament fraction. Consistent with this, Fhos2 localizes to the nestin intermediate filament but not to other cytoskeletons, as demonstrated by staining of H9c2 (2-1) cells with anti-Fhos2 antibodies. Furthermore, Fhos2 is present in nestin-expressing neuroepithelial cells of the fetal rat brain. Thus, Fhos2 not only has the actin-organizing activity but also associates with nestin, which may imply a Fhos2-mediated link between the nestin intermediate filament and actin microfilament.
机译：Fhos1是哺乳动物的FORMIN家族蛋白，可充当肌动蛋白微丝的组织者。在这里，我们已经克隆了人类和小鼠的cDNA，以查找名为Fhos2的新型Fhos同源物。 Fhos2的信息在心脏，肾脏和大脑中表达，而Fhos1 mRNA并不丰富。 Fhos2的两个剪接变体以组织特异性的方式存在。较长的变体Fhos2L是心脏的主要形式，而肾脏和大脑则主要表达编码较短蛋白质的Fhos2S。两种Fhos2变体以及Fhos1的活性形式的过表达诱导HeLa细胞中肌动蛋白应激纤维的形成，表明Fhos2充当肌动蛋白组织蛋白。使用大鼠心肌H9c2（2-1）细胞进行的生化分析表明，内源性Fhos2富含中间丝部分。与此相一致，Fhos2定位在巢蛋白中间丝而不是其他细胞骨架上，如用抗Fhos2抗体对H9c2（2-1）细胞染色所证明的那样。此外，Fhos2存在于胎鼠脑中表达巢蛋白的神经上皮细胞中。因此，Fhos2不仅具有肌动蛋白组织活性，而且与巢蛋白缔合，这可能暗示着巢蛋白中间丝与肌动蛋白微丝之间存在Fhos2介导的联系。

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