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首页> 外文期刊>Genome >DNA barcoding the Lepidoptera inventory of a large complex tropical conserved wildland, Area de Conservacion Guanacaste, northwestern Costa Rica
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DNA barcoding the Lepidoptera inventory of a large complex tropical conserved wildland, Area de Conservacion Guanacaste, northwestern Costa Rica

机译：DNA编码大型复杂热带保护野生动物鳞翅目的鳞翅目，哥斯达黎加西北部瓜纳卡斯特地区自然保护区

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The 37-year ongoing inventory of the estimated 15 000 species of Lepidoptera living in the 125 000 terrestrial hectares of Area de Conservacion Guanacaste, northwestern Costa Rica, has DNA barcode documented 11 000+ species, and the simultaneous inventory of at least 6000+ species of wild-caught caterpillars, plus 2700+ species of parasitoids. The inventory began with Victorian methodologies and species-level perceptions, but it was transformed in 2004 by the full application of DNA barcoding for specimen identification and species discovery. This tropical inventory of an extraordinarily species-rich and complex multidimensional trophic web has relied upon the sequencing services provided by the Canadian Centre for DNA Barcoding, and the informatics support from BOLD, the Barcode of Life Data Systems, major tools developed by the Centre for Biodiversity Genomics at the Biodiversity Institute of Ontario, and available to all through couriers and the internet. As biodiversity information flows from these many thousands of undescribed and often look-alike species through their transformations to usable product, we see that DNA barcoding, firmly married to our centuries-old morphology-, ecology-, microgeography-, and behavior-based ways of taxonomizing the wild world, has made possible what was impossible before 2004. We can now work with all the species that we find, as recognizable species-level units of biology. In this essay, we touch on some of the details of the mechanics of actually using DNA barcoding in an inventory.
机译：哥斯达黎加西北部瓜纳卡斯特地区12.5万公顷陆地上估计有15,000种鳞翅目的37年持续清册记录了DNA条形码，记录了11,000多种，同时至少有6000余种捕获的野生毛毛虫，以及2700多种寄生虫。该清单始于维多利亚时代的方法论和物种层面的认识，但在2004年通过全面应用DNA条形码进行标本识别和物种发现而进行了改造。热带地区丰富的物种多样性和复杂的多维营养网清单依赖于加拿大DNA条形码中心提供的测序服务，以及生命科学数据系统条形码BOLD的信息学支持，以及该中心开发的主要工具。安大略省生物多样性研究所的生物多样性基因组学，并通过快递和互联网提供给所有人。随着生物多样性信息从数千种未描述且通常相似的物种通过向可用产品的转化而流过，我们看到DNA条形码与我们已有数百年历史的形态学，生态学，微地理学和基于行为的方式牢固地结合在一起对野生生物进行分类的研究使2004年之前不可能实现的事情成为可能。我们现在可以将所有发现的物种作为可识别的物种级生物学单位进行研究。在本文中，我们将详细介绍在清单中实际使用DNA条形码的机制。

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